Click image to see more details
Product Info Summary
| SKU: | M00195 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ITCH Monoclonal Antibody
SKU/Catalog Number
M00195
BM5229 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ITCH Monoclonal Antibody catalog # M00195. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ITCH Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00195)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACBH-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ITCH
Reactive Species
M00195 is reactive to ITCH in Human, Mouse, Rat
Observed Molecular Weight
103 kDa
Calculated molecular weight
102.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00195 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of ITCH expression in K562 cell lysate.
Specific Publications For Anti-ITCH Monoclonal Antibody (M00195)
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Customer Q&As
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6 Customer Q&As for Anti-ITCH Monoclonal Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-ITCH Monoclonal antibody M00195. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-08
Question
I was wanting to use your anti-ITCH Monoclonal antibody for WB for mouse fetal brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse fetal brain identification?
Verified Customer
Verified customer
Asked: 2019-01-30
Answer
You can see on the product datasheet, M00195 anti-ITCH Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-30
Question
Is a blocking peptide available for product anti-ITCH Monoclonal antibody (M00195)?
Verified Customer
Verified customer
Asked: 2018-01-30
Answer
We do provide the blocking peptide for product anti-ITCH Monoclonal antibody (M00195). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-01-30
Question
Will anti-ITCH Monoclonal antibody M00195 work on pig WB with trachea?
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
Our lab technicians have not validated anti-ITCH Monoclonal antibody M00195 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ITCH Monoclonal antibody M00195 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig trachea in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-20
Question
See below the WB image, lot number and protocol we used for fetal brain using anti-ITCH Monoclonal antibody M00195. Please let me know if you require anything else.
P. Wu
Verified customer
Asked: 2013-11-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-01
Question
Would anti-ITCH Monoclonal antibody M00195 work for WB with fetal brain?
M. Parker
Verified customer
Asked: 2013-08-14
Answer
According to the expression profile of fetal brain, ITCH is highly expressed in fetal brain. So, it is likely that anti-ITCH Monoclonal antibody M00195 will work for WB with fetal brain.
Boster Scientific Support
Answered: 2013-08-14
