Anti-Phospho-AIP4 (Y420) ITCH Antibody

Product Name

Anti-Phospho-AIP4 (Y420) ITCH Antibody

View all ITCH/AIP4 Antibodies

SKU/Catalog Number

A00195Y420

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho-AIP4 (Y420) ITCH Antibody catalog # A00195Y420. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-AIP4 (Y420) ITCH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00195Y420)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human AIP4 around the phosphorylation site of Y420.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00195Y420 is reactive to Itch in Human, Mouse

Applications

A00195Y420 is guaranteed for ELISA, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

98.994kDa

Background of ITCH/AIP4

FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Sekine S et,al. (2008) J Immunol. ;180(12):8126-34
Pratz K et,al. (2008) Leuk Lymphoma. 2008 ;49(5):852-63.
Al Shaer L et,al. (2008) Br J Haematol. 141(4):483-93.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC 1:100-1:300
ELISA 1:20000

Validation Images & Assay Conditions

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Figure 1. Immunohistochemistry validation of ITCH using Anti-Phospho-AIP4 (Y420) ITCH Antibody (A00195Y420).

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C

Gene/Protein Information For Itch (Source: Uniprot.org, NCBI)

Alternative Names

AIF4; AIP4; AIP4dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)); atrophin-1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; EC 6.3.2; EC 6.3.2.-; ITCH; itchy (mouse homolog) E3 ubiquitin protein ligase; itchy E3 ubiquitin protein ligase homolog (mouse); itchy homolog E3 ubiquitin protein ligase; NAPP1; NAPP1E3 ubiquitin-protein ligase Itchy homolog; NFE2-associated polypeptide 1; ubiquitin protein ligase ITCH Itch|6720481N21Rik, 8030492O04Rik, A, A130065M08, AIP4, C230047C07Rik|itchy, E3 ubiquitin protein ligase|E3 ubiquitin-protein ligase Itchy|HECT-type E3 ubiquitin transferase Itchy homolog

For more info on Itch, check out the Itch Infographic

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In this infographic, you will see the following information for Itch: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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