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Product Info Summary
| SKU: | A00195 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ITCH Antibody
SKU/Catalog Number
A00195
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ITCH Antibody (Catalog # A00195). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ITCH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-ITCH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00195)
Host
Rabbit
Contents
ITCH antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ITCH antibody was raised against a 15 amino acid peptide near the amino terminus of human ITCH . The immunogen is located within amino acids 40 - 90 of ITCH.
Cross-reactivity
ITCH antibody is human, mouse and rat reactive. At least three isoforms of ITCH are known to exist; this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.
Reactive Species
A00195 is reactive to ITCH in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
102.8 kDa
Background of ITCH
The Itchy E3 ubiquitin protein ligase (ITCH) is a member of the Nedd4 family of HECT domain E3 ubiquitin ligases (1). HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. ITCH plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses (2). In B cells, ITCH is thought to associate with latent membrane protein 2A (LMP2A) of Epstein-Barr virus, specifically down-regulating its activity in B cell signaling (3). Mutations in this gene are a cause of syndromic multisystem autoimmune disease (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00195 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ITCH antibody can be used for detection of ITCH by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ITCH in 3T3 cell lysate with ITCH antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Specific Publications For Anti-ITCH Antibody (A00195)
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