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- Table of Contents
ITCH antibody (A00195) performs well in ELISA, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of ITCH in 3T3 cell lysate with ITCH antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Anti-ITCH Antibody
ITCH antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, WB |
Recommend Dilution
Application
Dilution
Species
ITCH antibody can be used for detection of ITCH by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-ITCH Antibody
View all ITCH Antibodies
View all ITCH Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
ITCH antibody can be stored at 4°C, stable for up to one year.
Host
Rabbit
Contents
ITCH antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ITCH antibody was raised against a 15 amino acid peptide near the amino terminus of human ITCH . The immunogen is located within amino acids 40 - 90 of ITCH.
Cross-Reactivity
ITCH antibody is human, mouse and rat reactive. At least three isoforms of ITCH are known to exist; this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
102.8 kDa
Background of ITCH
The Itchy E3 ubiquitin protein ligase (ITCH) is a member of the Nedd4 family of HECT domain E3 ubiquitin ligases (1). HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. ITCH plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses (2). In B cells, ITCH is thought to associate with latent membrane protein 2A (LMP2A) of Epstein-Barr virus, specifically down-regulating its activity in B cell signaling (3). Mutations in this gene are a cause of syndromic multisystem autoimmune disease (4).
Cite This Product
Anti-ITCH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00195)
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Product Promise
We promise Anti-ITCH Antibody performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00195
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026