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- Table of Contents
Facts about Integrin beta-2.
Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4.
Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen.Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain.
Human | |
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Gene Name: | ITGB2 |
Uniprot: | P05107 |
Entrez: | 3689 |
Belongs to: |
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integrin beta chain family |
959 beta subunit precursor)10; CD18 antigen; CD18; CD18beta 2; cell surface adhesion glycoprotein (LFA-1; CR3; Integrin beta 2; integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 beta; LFA-1; MAC-1 beta; MF17; MFI7; P150; p150,95 beta
Mass (kDA):
84.782 kDA
Human | |
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Location: | 21q22.3 |
Sequence: | 21; NC_000021.9 (44885949..44928815, complement) |
Leukocytes (PubMed:23775590). Expressed in neutrophils (at protein level) (PubMed:21193407, PubMed:28807980).
Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein.
When using the ITGB2 marker in your experiments, you could be wondering what the ideal application is for your experiment. It depends on your particular needs. We'll be discussing the specifications, applications, as well as troubleshooting of the ITGB2 marker in this article. For the best results, you must optimize your experiments. It is essential to identify the most common causes of error in experiments, and then figure out how to minimize these.
Boster Bio's Anti-CD18/ITGB2 Markers are derived from the human antibody polyclonal CD18. This antibody is available by catalog number PA1124. It is specifically designed to react with Human cells. This product is stable at -20°C over a year, or at -80degC for six months. The product has a sequence that is unique to human cells.
The ITGB2 gene encodes beta-2 integrin chains and is expressed on specific T cells. It is found on both BM and CAF cells. The ITGB2 marker was discovered by researchers at R&D in 2001. The ITGB2 marker's specifications include its location on the genome, its size and its functional annotations. It was recently approved by the Food and Drug Administration.
The expression of ITGB2 is present in the microenvironment of the tumor. It is found in both stromal and tumor cells in prostate, breast, ovarian, and colon cancers. Higher levels of the ITGB2 marker in CAFs was linked to more TNMs and an increased DOI. It was also linked with increased Ki67-positive cells. It isn't known whether ITGB2 can be expressed by cancer-associated fibrills.
The ITGB2 marker stimulates glycolysis in the mitochondria. This is accomplished through PI3K/AKT/mTOR signaling. This is a powerful therapeutic target. Cancer cells with high levels of ITGB2 are likely to have an increased oxidative phosphorylation. A higher amount of ITGB2 within cancer cells can enhance proliferative ability and inhibits metformin-resistant cell growth.
ITGB2 expression has been linked with increased MCT1 expression in cancer cells. This gene is also implicated in the development of insulin-resistant diabetes. It is possible that type 2 diabetes could be caused by the high levels of ITGB2 expression in cancer cells. When cultivated with CAFs that express ITGB2 cancer cells, they are also sensitive to metformin. The ITGB2 marker could be a therapeutic target.
The ITGB2 gene is responsible for the protein CD18. The protein has two functions: it acts as a cell-surface protein that binds the extracellular matrix. Cellular adhesion is a crucial process that contributes to extravasation from blood vessels. The ITGB2 gene can be found on the chromosome 21q22.3. The gene encodes a beta-chain integrin that forms heterodimers with alpha chains. Diseases associated with ITGB2 include Leukocyte Adhesion Deficiency, and Pertussis. The ITGB2 pathway is involved with a variety of processes, including adhesion as well as the degradation of the extracellular matrix.
To understand how ITGB2-AS1 influences cell proliferation, we used MCF-7 cells as well an MDA-MB-231 cell line. Q-PCR was used to verify the transfer of the virus ITGB2-AS1. Flow analysis and MTT assays revealed that the overexpression group showed higher rates of migration than the control group. We also utilized transwell assays to measure the rate of cell invasion and migration.
It is an integrin that regulates cell migration and invasion. It is believed to be involved in the growth of tumors as well as leukocytes. We created a plasmid that is carrying the ITGB2-AS1 genes to increase the expression of this gene in cancer cells. The plasmid was transfected in order to increase the expression of the ITGB2-AS1 gene without affecting the cell cycle distribution. ITGB2-AS1 expression increased cell migration. ITGB2-AS1 overexpression triggered activation of FAK/MMP9 signaling.
In the field of cancer research, the expression of ITGB2-AS1 in different types of breast tissue can be used to distinguish cancerous cells according to their ER/PR status. There are no treatment options currently available for breast cancers based on data from ITGB2-AS1 and a study of T2D is needed to determine whether the ITGB2 gene is involved in these kinds of disease. These studies demonstrate that ITGB2-AS1 is linked to the expression levels of the HER2 gene.
The ITGB2 gene carries two mutations, G400A and C562T. Both mutations increase the risk of metastasis from cancer by causing tumor cells expand and spread. The desmoplastic microenvironment of the PDAC includes the ITGB2 gene. ECM is a multidimensional, three-dimensional structure composed of structural and non-structural proteins. ECM proteins made from cancer cells play a crucial part in metastatic spread and in PDAC progression.
There are a few things to consider in order to solve the Boster Bio ITGB2-based assay. This marker is only activated by extracellular stimuli. It is not activated by signaling pathways that are "inside-out". The antibodies used in the Boster Bio ITGB2-based test are made of mice and rabbit, which are compatible with flow cytometry.
PMID: 3028646 by Kishimoto T.K., et al. Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family.
PMID: 1683838 by Weitzman J.B., et al. The gene organisation of the human beta 2 integrin subunit (CD18).
*More publications can be found for each product on its corresponding product page