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Product Info Summary
| SKU: | M00458-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse |
| Host: | Rat |
| Application: | Flow Cytometry, IP, IHC-P, IHC-F, WB |
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Product info
Product Name
Anti-Ms CD18 Purified Itgb2 Monoclonal Antibody
SKU/Catalog Number
M00458-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ms CD18 Purified Itgb2 Monoclonal Antibody (Catalog# M00458-3). Tested in Flow Cytometry, IP, WB, IHC-P, IHC-F application(s). This antibody reacts with Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Ms CD18 Purified Itgb2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00458-3)
Host
Rat
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
M18/2
Isotype
Rat IgG2a
Immunogen
Murine cytotoxic T cell glycoproteins. The rat monoclonal antibody M18/2 recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
Reactive Species
M00458-3 is reactive to Itgb2 in Mouse
Observed Molecular Weight
19 kDa
Calculated molecular weight
85.0 kDa
Background of Itgb2
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00458-3 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1 μg/ml.
Validation Images & Assay Conditions
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Separation of murine myeloid cells stained using anti-mouse CD18 (M18/2) purified antibody (concentration in sample 16 µg/ml, DAR APC, red-filled) from murine myeloid cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension.
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Flow cytometry surface staining pattern of murine splenocytes stained using anti-mouse CD18 (M18/2) purified antibody (concentration in sample 16 µg/ml) DAR APC.
Specific Publications For Anti-Ms CD18 Purified Itgb2 Monoclonal Antibody (M00458-3)
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