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- Table of Contents
2 Citations 4 Q&As
1 Citations
Facts about BDNF/NT-3 growth factors receptor.
Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that govern distinct overlapping signaling cascades.
Human | |
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Gene Name: | NTRK2 |
Uniprot: | Q16620 |
Entrez: | 4915 |
Belongs to: |
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protein kinase superfamily |
BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B
Mass (kDA):
91.999 kDA
Human | |
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Location: | 9q21.33 |
Sequence: | 9; NC_000009.12 (84668458..85027070) |
Isoform TrkB is expressed in the central and peripheral nervous system. In the central nervous system (CNS), expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophthalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is mainly expressed in the brain but also detected in other tissues including pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in the brain.
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Early endosome membrane. Cell projection, axon. Cell projection, dendrite. Cytoplasm, perinuclear region. Cell junction, synapse, postsynaptic density. Internalized to endosomes upon ligand-binding.
If you're interested in using the boster bio-anti-NTRK2 antibody for analyzing the expression of the protein, read on! This article will cover the application of this antibody as well as ELISA kits to determine the presence of NTRK2, PTN, and NTN1. Learn about the validation process and its applications. The NTRK2 marker is present in all mammals, including humans.
The anti-NTRK2 antigen from Boster bioengineering has been a breakthrough instrument for the research community. The company is located in Wuhan, PR China. Its NTRK2 marker antibody is made up of rabbit polyclonal antibodies to TrkB and Twist. This polyclonal antibody recognizes both NTRK2 and Twist. It was dilute to 1:1000.
A small amount of human brain tissue was taken to test the antibody. The samples were later processed using ELISA kits which measure BDNF, pro BDNF PKB/Akt, and Ntrk2. Following a pre-incubation phase followed by the samples being placed on an enzyme-labeled plates for 1.5 h at 37degC. The primary antibody was then diluted according to the manufacturer's instructions. To detect BDNF an additional antibody was employed.
ELISA kits for detection of NTRKS1, NTN1 and PTN are available in the market. These kits are designed to measure the presence of NTN1 in biological samples. The test has high sensitivity and specificity. The Ntn1 ELISA kit has good reproducibility. There is no cross-reactivity between the kit and any analogs. The kits should be stored between 2 and 8 degrees C.
The ELISA test is extremely sensitive and specific. It allows researchers to determine the amount of analytes in the sample. Since it makes use of highly specific antibodies, it is able to identify bound and unbound analyte and eliminate nonspecific substances. The sensitivity of the detection kit is easily compared with other methods.
ELISA kits for the detection of NTRKS, PTN1 and NTN1 offer a variety of applications. For instance the sandwich ELISA can detect NTN1 in a sample with no prior screening. There are ELISA kits for detection of PTN and NTN1 available for both clinical trials and research. They can detect the cell components in air, water and food items.
Standard ELISA assay formats include direct-adsorption and sandwich-ELISA. The nature of the signal detected will impact the steps used for detection and capture. A colorimetric signal can be identified using flat bottom plates made of polystyrene, which are clear, while fluorescent signals are detected using black or white opaque plates. A low CV value is required for reliable results.
The significance of NTRK2 is in its connection to general mental processes. It is also involved in psychopathology. In this study, researchers discovered an association between the NTRK2 genotype and emotional arousal. The results support the theory that NTRK2 is involved in processing emotion. These findings will require further research. In the meantime it is possible to identify mental illnesses by determining whether you are genetically predisposed to the condition.
The NTRK2 gene belongs to the NTRK family. Neurotrophins are the receptors that activate this membrane. This signaling is the reason for cell differentiation. Genetic mutations in this gene could cause obesity or other mood disorders. Additionally, this gene is subject to alternative splicing that can result in multiple transcript variants. These variants are typically identified by an distinct color.
The Y-axis depicts the linkage disequilibrium structure (LD) of the NTRK2 locus. Individuals with identical SNP genotypes could be linked. Genetic variations in the NTRK2 gene could affect GFR in certain individuals. Genetic studies using the NTRK2 gene as a marker are vital for developing new drugs. This marker is currently being developed for these purposes.
The NTRK2 protein is involved in the development and maintenance of the central nervous system. It regulates neuron survival, growth, differentiation, migration, and synapse formation. It may also play a role in determining the functions of certain brain areas. It has been shown that NTRK2 gene transcription has a positive correlation to depression. Interestingly, NTRK2 can be a marker for certain brain disorders, including schizophrenia, which are characterized by diminished cognitive function.
The confirmation of the NTRK2 marker is based on the identification of the top 350 genes that are highly related to the expression of the gene in GBM and LGG tissues. The genes play a role in morphogenesis, translation control and endocytic compartments. They also play a role in vesicular transport. NTRK2 is also a major factor in synaptic plasticity complex learning and synaptic plasticity. To validate the NTRK2 marker researchers must examine further to determine if the gene's expression is linked to these processes.
The gene encodes for a variety of transcripts that are located across the chromosome 9. The first 5' exons are complex and exhibit extensive patterns of splicing. They are an internal ribosomal entry site and translational start site. Later exons map onto the extracellular domain. The majority of 3' exons are located in the intron 14 of full-length NTRK2.
We identified a set of genes associated in GBM with NTRK2 expression by using an array of SNPs. In each group, we found two variants related to genes (BCR–NTRK2-NTRK2) Additionally, we identified genes that are associated with the NTRK2 marker that includes its coding area. It is interesting to note that the NTRK2 gene fusion in the GBM group was found to be associated with an increased chance of developing the disease.
The NTRK2-PML gene fusion has been identified in pilocytic astrocytoma. This mutation combines NTRK2 and PML genes, creating the possibility of developing cancer. Furthermore, the NTRK2-PML gene is associated with 12 regulatory motifs, and is associated with expression-enhancing chromatin changes in the frontal cortex and hippocampus.
There has been a correlation between the NTRK2 marker and the possibility of developing PTSD. This epigenetic marker is located in the 5' enhancer area of the gene. It was discovered to be associated with African individuals with a lower lifetime risk of developing PTSD. These findings do not support the notion of NTRK2 being a valuable method for diagnosing PTSD. Further research is required to better understand the connection between NTRK2 markers and PTSD.
The NTRK2 gene is involved in emotional processing and is involved in a variety of psychiatric disorders. It is linked to brain white matter and has been linked with emotional. The NTRK2 marker can be used to determine the likelihood of developing certain psychiatric disorders. However, more research is required to fully understand the way NTRK2 affects emotional processing.
The presence of the NTRK2 gene is associated with multiple neurodegenerative disorders, including Alzheimer's disease, autism, and epileptic and developmental encephalopathy. It is also associated with major depression disorders, as well as morbid obesity. There are orthologs to the NTRK2 gene in mice and humans. In both species, the gene is involved in the signaling pathways for neurotrophic factors. It interacts with neurotrophic factors and the drugs 17,alpha-ethynyl-estradiol and 2,3,7-tetrachlorodibenzene.
The development and maturation process of the central nervous systems is affected by the NTRK2 gene. It regulates neuron survival, proliferation, migration and differentiation, synapse development and plasticity. It is also found in peripheral nerves. However, the association between depression and NTRK2 is not fully understood. In addition to this study, NTRK2 markers are important in the diagnosis of depression in children.
PMID: 7789988 by Nakagawara A., et al. Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2).
PMID: 7823156 by Shelton D.L., et al. Human trks: molecular cloning, tissue distribution, and expression of extracellular domain immunoadhesins.
*More publications can be found for each product on its corresponding product page