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- Table of Contents
Ntrk2 antibody (M01388-3) performs well in IP, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Western blot analysis of TrkB using anti-TrkB antibody (M01388-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TrkB antigen affinity purified monoclonal antibody (Catalog # M01388-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TrkB at approximately 92,140 kDa. The expected band size for TrkB is at 92 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TrkB antigen affinity purified monoclonal antibody (Catalog # M01388-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TrkB at approximately 92,140 kDa. The expected band size for TrkB is at 92 kDa.
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Anti-TrkB Rabbit Monoclonal Antibody
Ntrk2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | IP, IHC, WB |
Tested Applications
Ntrk2 antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
IP 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IP
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-TrkB Rabbit Monoclonal Antibody
View all NTRK2 Antibodies
View all NTRK2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
22N08
Isotype
IgG
Immunogen
A synthesized peptide derived from human TrkB
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120-140 kDa
Calculated Molecular Weight
92.1 kDa
Cite This Product
Anti-TrkB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01388-3)
Protocols
Western Blot protocol for Ntrk2 antibody
Publications
Application:
Species:
Brain-derived neurotrophic factor in the ventrolateral orbital cortex regulates spared nerve injury-induced neuropathic pain and related emotional disorders in mice
Published: 2025-08-20
Journal: NEUROSCIENCE
Application: WB
Impact Factor: 2.80
Species: Mouse
PMID: 40846211
Identification and Validation of Antidepressant Small Molecules Using Bioinformatics and Mouse Depression Models
Published: 2025-08-20
Journal: Drug Design Development and Therapy
Application: WB
Impact Factor: 5.10
Species: Mouse
PMID: 40859969
Comparison of CUMS at different pregnancy stages, maternal separation, and their effects on offspring in postpartum depression mouse models
Published: 2024-07-27
Journal: Heliyon
Application: WB
Impact Factor: 3.40
Species: Mouse
PMID: 39166014
Esketamine Prevents Postoperative Emotional and Cognitive Dysfunction by Suppressing Microglial M1 Polarization and Regulating the BDNF-TrkB Pathway in Ageing Rats with Preoperative Sleep Disturbance
Published: 2024-01-15
Journal: MOLECULAR NEUROBIOLOGY
Application: WB
Impact Factor: 4.60
Species: Rat
PMID: 38221533
Differential expression of microRNAs in TM3 Leydig cells of mice treated with brain-derived neurotrophic factor
Published: 2017-10-02
Journal: CELL BIOCHEMISTRY AND FUNCTION
Application: IF
Impact Factor: 2.19
Species: Mouse
PMID: 28967682
The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm
Published: 2011-10-18
Journal: THERIOGENOLOGY
Application: WB,IF
Impact Factor: 2.05
Species: Cow
PMID: 22015157
Icariin isolated from Epimedium brevicornum Maxim attenuates learning and memory deficits induced by d-galactose in rats
Published: 2010-06-01
Journal: PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
Application: WB
Impact Factor: 2.97
Species: Rat
PMID: 20566405
Review
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1 Review For Anti-TrkB Rabbit Monoclonal Antibody
The Anti-TrkB antibody (M01388-3) produced a clear and specific target band at the expected molecular weight with low background in normal mouse hippocampal tissue by Western blot, demonstrating reliable performance.
| SKU | M01388-3 |
|---|---|
| Application | Western Blot |
| Sample | mouse hippocampal tissue |
| Sample Processing Description | The left hippocampus was dissected from normal mouse brains, and total protein was extracted. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | TrkB Rabbit Monoclonal Antibody |
| Primary Incubation | 1:3000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | Tropomyosin receptor kinase B (TrkB) is an essential mediator of BDNF-induced long-term potentiation (LTP) and spatial memory formation, playing a central role in neural development and synaptic plasticity. In this study, hippocampal tissues from two normal mice were used to evaluate the performance of the Anti-TrkB antibody. The results showed a clear target band at the expected molecular weight, indicating that the antibody performs well and provides reliable detection in Western blot. |
Yajun Qiao, Qinghai University
Verified customer
Submitted 2026-07-06
Product Promise
We promise Anti-TrkB Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M01388-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Boster Guarantee
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026