Mouse TrkB ELISA Kit PicoKine™

Mouse TrkB ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ntrk2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 5pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0849
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Product Name

Mouse TrkB ELISA Kit PicoKine™

See all TrkB products

SKU/Catalog Number

EK0849

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TrkB ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ntrk2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse TrkB ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0849)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: C32-H429

Sensitivity

<5 pg/ml

Assay Range

93.8 pg/ml - 6,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0849 is reactive to Ntrk2 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

EK0849 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0849-CAP Anti-Mouse Ntrk2 Pre-coated 96-well strip microplate 1
EK0849-ST Mouse Ntrk2 Standard 2 vials, 10 ng/tube
EK0849-DA Mouse Ntrk2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)093.8187.5375750150030006000
O.D.0.0080.0810.1690.2980.6121.0001.5612.204

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TrkB, Dilution ratio of 1:1, concentration in serum is 2-5ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TrkB ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)18867920082026222201
Standard deviation12.2246.85128.5113.7346.02149.66
CV (%)6.5%6.9%6.4%6.8%7.4%6.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TrkB in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11881871952011925.672.9%
Sample 267978166267669947.486.7%
Sample 32008203721962162210079.823.8%
*number of samples for each test n=16.

Gene/Protein Information For Ntrk2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P15209

Gene ID

18212

Gene Name

Ntrk2

Full Name

BDNF/NT-3 growth factors receptor

Weight

92.133kDa

Superfamily

protein kinase superfamily

Alternative Names

BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B Ntrk2|GP145-TrkB/GP95-TrkB, Tkr, Tkrb, trk, trk-B, trkB|neurotrophic tyrosine kinase, receptor, type 2|BDNF/NT-3 growth factors receptor|neurotrophic tyrosine receptor kinase type 2|trkB tyrosine kinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ntrk2, check out the Ntrk2 Infographic

Ntrk2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ntrk2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0849 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalities
Species: Mouse

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4 Customer Q&As for Mouse TrkB ELISA Kit PicoKine™

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

L. Kulkarni

Verified customer

Asked: 2020-12-09

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-12-09

Question

Q: is it okay to use citrate plasma as samples in Mouse TrkB Picokine® ELISA Kit (Catalog # EK0849)?

Verified Customer

Verified customer

Asked: 2020-09-12

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of TrkB causing disruption of its protein structure. TrkB may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TrkB ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-09-12

Question

Q: if the enzyme conjugated TrkB antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TrkB antigen?

Verified Customer

Verified customer

Asked: 2020-06-22

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-06-22

Question

Q: how to analyze ELISA data? I have obtained TrkB level in serum.

Verified Customer

Verified customer

Asked: 2018-08-28

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-08-28

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