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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)|
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Rat TRKB ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat TRKB ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ntrk2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml .
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat TRKB ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1596)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: C32-H429
93.6 pg/ml - 6,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1596 is reactive to Ntrk2 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
EK1596 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1596-CAP||Anti-Rat Ntrk2 Pre-coated 96-well strip microplate||1|
|EK1596-ST||Rat Ntrk2 Standard||2 vials, 10 ng/tube|
|EK1596-DA||Rat Ntrk2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat TRKB PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TrkB, Dilution ratio of 1:1, concentration in serum is 200-500pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat TRKB ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of TrkB in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Ntrk2 (Source: Uniprot.Org, NCBI)
BDNF/NT-3 growth factors receptor
protein kinase superfamily
BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 126.96.36.199; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B Ntrk2|RATTRKB1, TRKB1, Tkrb, trk-B, trkB|neurotrophic receptor tyrosine kinase 2|BDNF/NT-3 growth factors receptor|GP145-TrkB/GP95-TrkB|neural receptor protein-tyrosine kinase (trkB)|neurotrophic tyrosine kinase, receptor, type 2|neurotrophic tyrosine receptor kinase type 2|trkB tyrosine kinase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ntrk2, check out the Ntrk2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ntrk2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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