PDGFRA Antibodies

Platelet-derived growth factor receptor alpha (PDGFRA)

Tyrosine-protein kinase that functions as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Based on the circumstance, promotes or inhibits cell proliferation and cell migration.

Plays an essential role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton growth and cephalic closure during embryonic development.

Gene Information

Human
Gene Name:	PDGFRA
Uniprot:	P16234
Entrez:	5156

Family

Belongs to:
protein kinase superfamily

Alternative Names

alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

Molecular Weight

Mass (kDA):
122.67 kDA

Genomic Context

Human
Location:	4q12
Sequence:	4; NC_000004.12 (54229127..54298247)

Tissue Specificity

Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein. Cell projection, cilium. Golgi apparatus.

Diseases

• Neoplasms
• Gastrointestinal Stromal Tumors
• Malignant Neoplasms
• Stromal Neoplasm
• Malignant Paraganglionic Neoplasm
• Neoplasm Metastasis
• Leukemia
• Eosinophilia
• Hypereosinophilic Syndrome
• Sarcoma
• Carcinoma
• Stomach Neoplasms
• Gastrointestinal Neoplasms

• Myeloid Leukemia
• Cell Transformation, Neoplastic
• Myeloproliferative Disease
• Brain Neoplasms
• Idiopathic Hypereosinophilic Syndrome
• Glioblastoma
• Eosinophilic Leukemia

Interacting Proteins

• CCDC155
• CRK
• CRKL
• EGFR
• PDGFA

• PDGFB
• PDGFC
• PDGFRB

Pathways

• Pathogenesis
• Localization
• Cell Proliferation
• Cell Growth
• Drug Resistance
• Oncogenesis
• Cell Cycle
• Methylation
• Reverse Transcription
• Angiogenesis
• Transport
• Cell Differentiation

• Interphase
• Dna Methylation
• Cell Adhesion
• Pigmentation
• Cell Death
• Translation
• Cell Migration

PTMs

• Phosphorylation
• Methylation
• Cleavage
• Dephosphorylation

• Demethylation
• Carboxylation
• Deacetylation
• Acetylation

• Oxidation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PDGFRA

Boster Bio - Best Uses Of The PDGFRA Marker

If you're a scientist you might want to find out more about the PDGFRA Marker and what applications it has. Listed below are the benefits of Boster Bio products. Scientists can submit their findings for specific species or samples in order to receive credit for the product. These benefits are accessible to scientists from all over the world. Continue reading to learn more about how you can reap these advantages.

PDGFRA Marker

PDGFRA is an amino acid that has both morphological and proliferative characteristics. It is utilized in numerous studies to determine the effects of tumor growth and chemotherapy. However, it is not completely understood how to utilize this marker in clinical trials. To understand the significance of this gene and its numerous uses, one has to first know the definition of PDGFRA. PDGFRA is a protein that can be broken down into several subtypes.

Si-PDGFRb

The PDGFRA gene is among the most frequently expressed proteins in the brain, and its knockout results provide new insights into how the brain process the signals from nutrients. PDGFRb is involved in the expression of various genes related to EMT. A decrease in the levels of the PDGFRA gene is linked to down-regulation of PDGFRb. These genes include a-SMA, Ccl12, and Snail.

HOS cells were transfected with the siRNA–PDGFRb gene sequence and incubated for an hour with the corresponding 10mL Cell Counting Kit-8. (CCK-8), Boster Bio, Wuhan. China. The results were analyzed using one-way ANOVA and Newman-Keuls posttest.

PDGFRb downregulation inhibited HOS cells migration and reduced the levels markers of stromal phenotypes. PDGF-BB also inhibited Akt the phosphorylation process, which could be useful in treating OS. These results suggest that PDGFRb may be a possible therapeutic target. Research: Best Uses of the PDGFRA marker

The PDGFRA is a key factor in the development and progression of OS. It is also recognized that it affects Akt and mTOR-mediated signaling. PDGFRb plays a role in the migration and invasion of HOS cells. The expression of PDGFRA hinders this process. Numerous research studies have proven that this gene may be involved in OS. The PDGFRA gene is now widely accessible.

Nontargeting control siRNA duplexes

Researchers can compare siRNA delivery to control conditions by using non-targeting siRNAs in RNAi studies. These reagents have the ability to limit off-target effects by targeting nonoverlapping regions of the target mRNA. These reagents are crucial for eliminating the target gene. Furthermore, they are highly effective in avoiding unwanted side effects.

Researchers utilized a web-based tool in an earlier study to examine the expression levels of their favorite genes against 53 different melanoma cell lines. To verify that the RNA was targeted, they validated the reagents for qRTPCR and western blot. There are some caveats with knockdown tests that could make interpretation challenging.

To maximize the conditions for transfection to maximize the transfection conditions, fluorescently labeled RNAs were used. These siRNAs are used for positive controls and also as a visual indicator of the performance of transfection. Transfection is achievable using Lipofectamine-RNAiMAX reagents. Apart from amplification of transfection, transfection reagents are essential to ensure the success of transfection.

Applications

This article will explore the role played by the PDGFRA marker in pulmonary diseases. We will also discuss the role of pulmonary fibrosis as well as how this gene contributes normal maturation to fibrosis. We will review the PDGFRA mutation landscape, its role as a marker of adult fibrosis and potential therapeutic applications. We provide an illustration of how we could use the PDGFRA marker to identify patients suffering from pulmonary fibrosis.

The PDGFRA marker plays a vital role in predicting the severity of immune infections in PDAC. This is associated with long OS. It is a good indicator of the response to therapy and targeting this protein could provide new treatment strategies. More research is required to discover the mechanism behind PDGFRA, and immune infiltration. A limited sample size may result in some bias due to the current study. Future clinical trials are needed to validate the results of this study.

Research has shown that PDGFRA is associated with various types of tumors and the majority of cancer-related disorders. For example, PDGFRA and EPHA2 have significant overlap in cancer as well as intestinal stromal tumors. PDGFRA and EPHA2 are co-upregulated and excessive levels of one or the other can lead to poor prognosis as well as therapeutic effects. This suggests that both of these proteins can be utilized in conjunction to detect tumor-related diseases.

Products

PDGF, a growth factor, has been implicated with wound healing. It is an attractant for chemoattractants in many processes such as the formation of bone, erythropoiesis, and wound healing. It is also involved in the normal development of the respiratory and cardiovascular systems. But what is the function of PDGF? What is its purpose?

The PDGFRA protein is made by a cell-based expression system. The PDGFRA molecule has an Fc tag at its C-terminus. The receptor binds the ligand, which phosphorylates the substrate. This triggers signaling proteins. The PDGFRA receptor is present in embryonic tissues as well as mesenchymal-derived adult cells.

PDGFRA expression in tumor cells is different from that of normal tissue. PDGFRb is expressed in tumor cells in more than two-thirds (33 percent) of cases. The tumor cells express this protein in greater numbers than those of normal tissues. Angiogenesis and tumor growth are aided by PDGF signaling. The cell's surface has the PDGF receptor. The PDGF receptor is used in two forms, alpha and beta. The alpha form is essential for kidney development. Mice carrying a homozygous PDGFRA gene have impaired kidney development.

Resources

There are a variety of sources for anti-PDGFRA antibodies. The antibodies have been evaluated for validity for over 25 years and are extensively used by the research community. They are made of polypeptides and recombinant proteins that target specific epitopes in the antigen. A large number of these antibodies are listed in the Boster database. These antibodies can be used in different ways such as clinical trials and cellular biology.