Click image to see more details
Product Info Summary
| SKU: | M00366 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PDGFR alpha Rabbit Monoclonal Antibody
SKU/Catalog Number
M00366
BM4982 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PDGFR alpha Rabbit Monoclonal Antibody catalog # M00366. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDGFR alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00366)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAOE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PDGFR alpha
Reactive Species
M00366 is reactive to PDGFRA in Human, Mouse, Rat
Observed Molecular Weight
180 kDa
Calculated molecular weight
122.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00366 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PDGFR alpha/PDGFRA using anti-PDGFR alpha/PDGFRA antibody (M00366).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat ovary tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse ovary tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFR alpha/PDGFRA antigen affinity purified monoclonal antibody (M00366) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDGFR alpha/PDGFRA at approximately 180 kDa. The expected band size for PDGFR alpha/PDGFRA is at 123 kDa.
Specific Publications For Anti-PDGFR alpha Rabbit Monoclonal Antibody (M00366)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PDGFR alpha Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-PDGFR alpha Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-PDGFR alpha Rabbit Monoclonal Antibody
Question
Does M00366 require dry ice during shipping?
Verified customer
Asked: 2021-10-20
Answer
We ship the Anti-PDGFR Alpha Rabbit Monoclonal Antibody (M00366) with gel ice.
Boster Scientific Support
Answered: 2021-10-21
Question
Our lab were happy with the WB result of your anti-PDGFR alpha Rabbit Monoclonal antibody. However we have seen positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is PDGFRA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
According to literature, placenta does express PDGFRA. Generally PDGFRA expresses in cell membrane. Regarding which tissues have PDGFRA expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8586421
Brain, Pubmed ID: 2536956
Colon tumor, Pubmed ID: 7896447
Eosinophil, Pubmed ID: 12808148
Foreskin, Pubmed ID: 2544881
Lung, and Trachea, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-26
Question
We are currently using anti-PDGFR alpha Rabbit Monoclonal antibody M00366 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
J. Parker
Verified customer
Asked: 2017-11-03
Answer
The anti-PDGFR alpha Rabbit Monoclonal antibody (M00366) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-03
Question
We have observed staining in human blood. Do you have any suggestions? Is anti-PDGFR alpha Rabbit Monoclonal antibody supposed to stain blood positively?
J. Jones
Verified customer
Asked: 2015-03-30
Answer
Based on literature blood does express PDGFRA. Based on Uniprot.org, PDGFRA is expressed in endometrium, foreskin, brain, blood, lung trachea, placenta, eosinophil, colon tumor, among other tissues. Regarding which tissues have PDGFRA expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8586421
Brain, Pubmed ID: 2536956
Colon tumor, Pubmed ID: 7896447
Eosinophil, Pubmed ID: 12808148
Foreskin, Pubmed ID: 2544881
Lung, and Trachea, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-03-30
