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Product Info Summary
| SKU: | A30446 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PDGFR alpha Antibody
SKU/Catalog Number
A30446
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PDGFR alpha Antibody (Catalog# A30446). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDGFR alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30446)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human PDGFR alpha. Immunogen sequence location: 731-780
Reactive Species
A30446 is reactive to PDGFRA in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
122.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30446 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of SH-SY5Y cells using PDGFR-α Polyclonal Antibody
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Immunofluorescence analysis of HeLa cells, using PDGFR alpha Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PDGFR alpha Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-PDGFR alpha Antibody (A30446)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-PDGFR alpha Antibody
Question
I see that the anti-PDGFR alpha antibody A30446 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-05-06
Question
See below the WB image, lot number and protocol we used for endometrium using anti-PDGFR alpha antibody A30446. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-19
Question
Is a blocking peptide available for product anti-PDGFR alpha antibody (A30446)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
We do provide the blocking peptide for product anti-PDGFR alpha antibody (A30446). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-15
Question
Will A30446 anti-PDGFR alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
You can see on the product datasheet, A30446 anti-PDGFR alpha antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-23
Question
I was wanting to use your anti-PDGFR alpha antibody for IHC for rat endometrium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat endometrium identification?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
It shows on the product datasheet, A30446 anti-PDGFR alpha antibody has been tested for IF, IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat endometrium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-09
Question
Is there a BSA free version of anti-PDGFR alpha antibody A30446 available?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PDGFR alpha antibody A30446 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-02
Question
My question regards using your anti-PDGFR alpha antibody for lung development studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
Thank you for your inquiry. This A30446 anti-PDGFR alpha antibody is tested on human brain tissue, hela cells. It is guaranteed to work for IF, IHC in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-24
Question
Will anti-PDGFR alpha antibody A30446 work for IHC with endometrium?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
According to the expression profile of endometrium, PDGFRA is highly expressed in endometrium. So, it is likely that anti-PDGFR alpha antibody A30446 will work for IHC with endometrium.
Boster Scientific Support
Answered: 2019-07-08
Question
We are currently using anti-PDGFR alpha antibody A30446 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
The anti-PDGFR alpha antibody (A30446) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-15
Question
We are interested in to test anti-PDGFR alpha antibody A30446 on rat endometrium for research purposes, then I may be interested in using anti-PDGFR alpha antibody A30446 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Wu
Verified customer
Asked: 2018-11-06
Answer
The products we sell, including anti-PDGFR alpha antibody A30446, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-06
Question
Is this A30446 anti-PDGFR alpha antibody reactive to the isotypes of PDGFRA?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
The immunogen of A30446 anti-PDGFR alpha antibody is A peptide derived from human PDGFR alpha. Immunogen sequence location: 731-780. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-15
Question
We have tried in the past anti-PDGFR alpha antibody for IF on lung trachea in the past. I am using mouse, and We intend to use the antibody for IHC next. I am interested in examining lung trachea as well as eosinophil in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
I took a look at the website and datasheets of our anti-PDGFR alpha antibody and it appears that A30446 has been tested on mouse in both IF and IHC. Thus A30446 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-03-01
Question
My boss were content with the WB result of your anti-PDGFR alpha antibody. However we have been able to see positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is PDGFRA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
Based on literature, placenta does express PDGFRA. Generally PDGFRA expresses in cell membrane. Regarding which tissues have PDGFRA expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8586421
Brain, Pubmed ID: 2536956
Colon tumor, Pubmed ID: 7896447
Eosinophil, Pubmed ID: 12808148
Foreskin, Pubmed ID: 2544881
Lung, and Trachea, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-02-12
Question
We have observed staining in human endometrium. Do you have any suggestions? Is anti-PDGFR alpha antibody supposed to stain endometrium positively?
A. Jackson
Verified customer
Asked: 2017-11-10
Answer
From what I have seen in literature endometrium does express PDGFRA. From what I have seen in Uniprot.org, PDGFRA is expressed in endometrium, foreskin, brain, blood, lung trachea, placenta, eosinophil, colon tumor, among other tissues. Regarding which tissues have PDGFRA expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8586421
Brain, Pubmed ID: 2536956
Colon tumor, Pubmed ID: 7896447
Eosinophil, Pubmed ID: 12808148
Foreskin, Pubmed ID: 2544881
Lung, and Trachea, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-11-10
Question
Can you help my question with product A30446, anti-PDGFR alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Gonzalez
Verified customer
Asked: 2016-06-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30446 anti-PDGFR alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-06-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for endometrium using anti-PDGFR alpha antibody A30446. Let me know if you need anything else.
K. Thomas
Verified customer
Asked: 2016-03-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-10
