Anti-PDGFR alpha Antibody

Boster Bio Anti-PDGFR alpha Antibody catalog # A30446. Tested in IF, IHC applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A30446
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC

Product Name

Anti-PDGFR alpha Antibody

See all PDGFR alpha products

SKU/Catalog Number

A30446

Size

100ul

Form

Liquid

Description

Boster Bio Anti-PDGFR alpha Antibody catalog # A30446. Tested in IF, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PDGFR alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30446)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

A peptide derived from human PDGFR alpha. Immunogen sequence location: 731-780

Reactive Species

A30446 is reactive to PDGFRA in Human, Mouse, Rat

Applications

A30446 is guaranteed for IF, IHC Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

User need to optimize the dilution ratio for this antibody

Validation Images & Assay Conditions

Gene/Protein Information For PDGFRA (Source: Uniprot.Org, NCBI)

Uniprot ID

P16234

Gene ID

5156

Gene Name

PDGFRA

Full Name

Platelet-derived growth factor receptor alpha

Weight

122.67kDa

Superfamily

protein kinase superfamily

Alternative Names

alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA PDGFRA CD140A, PDGFR-2, PDGFR2 platelet derived growth factor receptor alpha platelet-derived growth factor receptor alpha|CD140 antigen-like family member A|CD140a antigen|PDGF-R-alpha|alpha-type platelet-derived growth factor receptor|platelet-derived growth factor receptor 2|platelet-derived growth factor receptor, alpha polypeptide

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PDGFRA, check out the PDGFRA Infographic

PDGFRA infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PDGFRA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A30446 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

PDGFR%u03B1 regulated by miR-34a and FoxO1 promotes adipogenesis in porcine intramuscular preadipocytes through Erk signaling pathway

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16 Customer Q&As for Anti-PDGFR alpha Antibody

Question

I see that the anti-PDGFR alpha antibody A30446 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-05-06

Question

See below the WB image, lot number and protocol we used for endometrium using anti-PDGFR alpha antibody A30446. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-19

Question

Is a blocking peptide available for product anti-PDGFR alpha antibody (A30446)?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

We do provide the blocking peptide for product anti-PDGFR alpha antibody (A30446). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-15

Question

Will A30446 anti-PDGFR alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

You can see on the product datasheet, A30446 anti-PDGFR alpha antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-23

Question

I was wanting to use your anti-PDGFR alpha antibody for IHC for rat endometrium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat endometrium identification?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

It shows on the product datasheet, A30446 anti-PDGFR alpha antibody has been tested for IF, IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat endometrium in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-09

Question

Is there a BSA free version of anti-PDGFR alpha antibody A30446 available?

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PDGFR alpha antibody A30446 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-02

Question

My question regards using your anti-PDGFR alpha antibody for lung development studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

Thank you for your inquiry. This A30446 anti-PDGFR alpha antibody is tested on human brain tissue, hela cells. It is guaranteed to work for IF, IHC in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-24

Question

Will anti-PDGFR alpha antibody A30446 work for IHC with endometrium?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

According to the expression profile of endometrium, PDGFRA is highly expressed in endometrium. So, it is likely that anti-PDGFR alpha antibody A30446 will work for IHC with endometrium.

Boster Scientific Support

Answered: 2019-07-08

Question

We are currently using anti-PDGFR alpha antibody A30446 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-03-15

Answer

The anti-PDGFR alpha antibody (A30446) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-03-15

Question

We are interested in to test anti-PDGFR alpha antibody A30446 on rat endometrium for research purposes, then I may be interested in using anti-PDGFR alpha antibody A30446 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

K. Wu

Verified customer

Asked: 2018-11-06

Answer

The products we sell, including anti-PDGFR alpha antibody A30446, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-11-06

Question

Is this A30446 anti-PDGFR alpha antibody reactive to the isotypes of PDGFRA?

Verified Customer

Verified customer

Asked: 2018-08-15

Answer

The immunogen of A30446 anti-PDGFR alpha antibody is A peptide derived from human PDGFR alpha. Immunogen sequence location: 731-780. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-08-15

Question

We have tried in the past anti-PDGFR alpha antibody for IF on lung trachea in the past. I am using mouse, and We intend to use the antibody for IHC next. I am interested in examining lung trachea as well as eosinophil in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2018-03-01

Answer

I took a look at the website and datasheets of our anti-PDGFR alpha antibody and it appears that A30446 has been tested on mouse in both IF and IHC. Thus A30446 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-03-01

Question

My boss were content with the WB result of your anti-PDGFR alpha antibody. However we have been able to see positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is PDGFRA supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-02-12

Answer

Based on literature, placenta does express PDGFRA. Generally PDGFRA expresses in cell membrane. Regarding which tissues have PDGFRA expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8586421
Brain, Pubmed ID: 2536956
Colon tumor, Pubmed ID: 7896447
Eosinophil, Pubmed ID: 12808148
Foreskin, Pubmed ID: 2544881
Lung, and Trachea, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-02-12

Question

We have observed staining in human endometrium. Do you have any suggestions? Is anti-PDGFR alpha antibody supposed to stain endometrium positively?

A. Jackson

Verified customer

Asked: 2017-11-10

Answer

From what I have seen in literature endometrium does express PDGFRA. From what I have seen in Uniprot.org, PDGFRA is expressed in endometrium, foreskin, brain, blood, lung trachea, placenta, eosinophil, colon tumor, among other tissues. Regarding which tissues have PDGFRA expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8586421
Brain, Pubmed ID: 2536956
Colon tumor, Pubmed ID: 7896447
Eosinophil, Pubmed ID: 12808148
Foreskin, Pubmed ID: 2544881
Lung, and Trachea, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-11-10

Question

Can you help my question with product A30446, anti-PDGFR alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

L. Gonzalez

Verified customer

Asked: 2016-06-21

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30446 anti-PDGFR alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-06-21

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for endometrium using anti-PDGFR alpha antibody A30446. Let me know if you need anything else.

K. Thomas

Verified customer

Asked: 2016-03-10

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-03-10

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A30446
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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