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PDGFRA antibody (AZQ9DE49) performs well in IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of PDGFRA using anti-PDGFRA antibody (AZQ9DE49).
PDGFRA was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDGFRA Antibody (AZQ9DE49) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
PDGFRA was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDGFRA Antibody (AZQ9DE49) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish PDGFR alpha/PDGFRA Antibody
PDGFRA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC |
Tested Applications
PDGFRA antibody was positively detected by IHC in:
zebrafish brain tissue, zebrafish spinal cord tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish PDGFR alpha/PDGFRA Antibody
View all PDGFRA Antibodies
View all PDGFRA Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish PDGFR alpha/PDGFRA recombinant protein (Position: Y93-E469).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of PDGFRA
Predicted to enable platelet-derived growth factor alpha-receptor activity and platelet-derived growth factor binding activity. Acts upstream of or within several processes, including circulatory system development; embryonic neurocranium morphogenesis; and neural crest cell migration. Predicted to be located in Golgi apparatus and cilium. Predicted to be part of receptor complex. Predicted to be active in plasma membrane. Is expressed in several structures, including cardiovascular system; head; mesoderm; nervous system; and neural crest. Human ortholog(s) of this gene implicated in hematologic cancer (multiple); hypereosinophilic syndrome; and pancreatic cancer. Orthologous to human PDGFRA (platelet derived growth factor receptor alpha).
Cite This Product
Anti-Zebrafish PDGFR alpha/PDGFRA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ9DE49)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Mouse
41%
Low homology
Rat
40%
Low homology
Human
39%
Low homology
Monkey
39%
Low homology
Sheep
38%
Low homology
Dog
38%
Low homology
Cow
38%
Low homology
Rabbit
10%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for PDGFRA antibody
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Product Promise
We promise Anti-Zebrafish PDGFR alpha/PDGFRA Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
AZQ9DE49
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026