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Product Info Summary
| SKU: | AZQ9DE49 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish PDGFR alpha/PDGFRA Antibody
SKU/Catalog Number
AZQ9DE49
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish PDGFR alpha/PDGFRA Antibody catalog # AZQ9DE49. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish PDGFR alpha/PDGFRA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ9DE49)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish PDGFR alpha/PDGFRA recombinant protein (Position: Y93-E469).
Reactive Species
AZQ9DE49 is reactive to PDGFRA in Zebrafish
Background of PDGFRA
Predicted to enable platelet-derived growth factor alpha-receptor activity and platelet-derived growth factor binding activity. Acts upstream of or within several processes, including circulatory system development; embryonic neurocranium morphogenesis; and neural crest cell migration. Predicted to be located in Golgi apparatus and cilium. Predicted to be part of receptor complex. Predicted to be active in plasma membrane. Is expressed in several structures, including cardiovascular system; head; mesoderm; nervous system; and neural crest. Human ortholog(s) of this gene implicated in hematologic cancer (multiple); hypereosinophilic syndrome; and pancreatic cancer. Orthologous to human PDGFRA (platelet derived growth factor receptor alpha).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ9DE49 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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IHC analysis of PDGFRA using anti-PDGFRA antibody (AZQ9DE49).
PDGFRA was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDGFRA Antibody (AZQ9DE49) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of PDGFRA using anti-PDGFRA antibody (AZQ9DE49).
PDGFRA was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDGFRA Antibody (AZQ9DE49) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish PDGFR alpha/PDGFRA Antibody (AZQ9DE49)
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