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PRKCA / Protein kinase C alpha type · IHC design guide
Design Immunohistochemistry for PRKCA
Plan PRKCA staining in paraffin sections using cerebellar Purkinje cells as a high-expression reference (HPA tissue IHC). The IHC-validated antibody PB9318 has a reported working range of 0.5–1 μg/ml (datasheet: PB9318).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
PRKCA Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic in most tissues; Purkinje cells also show membrane staining (HPA tissue IHC) | |
| Staining pattern | Mostly cytoplasmic; Purkinje cells show cytoplasm/membrane staining (HPA tissue IHC) | |
| Antigen retrieval | Citrate pH 6 HIER, heat-mediated (datasheet PB9318) | |
| Positive control | Adrenal gland+4 more · see all | |
| Negative control | Breast+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep fixation consistent across compared paraffin sections. (selected-SKU IHC image PB9318) | |
| Caveat | Antibody staining has medium consistency with RNA expression (HPA tissue IHC) | |
| Regulation | CSF1-driven macrophage differentiation shifts PRKCA to the nucleus (UniProt) | |
| Isoform / epitope | 0 isoforms annotated; no signal peptide or transmembrane segment (UniProt) |
Section 1
Recommended PRKCA IHC & IF Protocols
Start with the catalog antibody’s IHC-P protocol (datasheet: PB9318), then compare published protocols for paraffin nucleus pulposus sections (PMC10249188) and rat brain sections (PMC10707860).
| Sample | Paraffin-embedded mouse intestine tissues; fixative not specified (datasheet PB9318) |
| Fixation | Image fixative and duration unreported (datasheet PB9318); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: Citrate pH 6, 20 min (datasheet PB9318) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet PB9318) |
| Primary antibody | Rabbit anti-PRKCA, 0.5-1μg/ml (datasheet PB9318) |
| Primary incubation | Overnight at 4 °C (datasheet PB9318) |
| Detection | Streptavidin-biotin complex (SABC), DAB chromogen (datasheet PB9318) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | PRKCA-positive staining in glandular cells of adrenal gland (HPA tissue IHC: High). HPA tissue profile: Cytoplasmic expression in most tissues, high expression in CNS. No signal in the no-primary control. |
💡Decision noteTry citrate pH 6 heat retrieval first (datasheet: PB9318); consider EDTA retrieval for nucleus pulposus sections if staining needs optimization (PMC10249188).
Section 2
What Is the Expected PRKCA Staining Pattern?
PRKCA staining is chiefly cytoplasmic across tissues, with high staining in cerebellar Purkinje cells, hippocampal neurons, and adrenal glandular cells (HPA tissue IHC: Enhanced; medium agreement with RNA). Membrane-associated staining is plausible, and nuclear localization is also reported (UniProt P17252: subcellular location). PRKCA has no transmembrane segment, so membrane staining does not imply an integral membrane protein (UniProt P17252: topology).
| Purkinje cell cytoplasm and membrane stain strongly; nearby cells differ in intensity. | This matches the high Purkinje cell signal recorded in cerebellum (HPA tissue IHC: High in Purkinje cell cytoplasm/membrane). Score identifiable cell types and compartments separately: a stained cerebellar section does not mean every cell should stain equally (HPA tissue IHC: cell-specific observations). |
| Hippocampal neurons or adrenal glandular cells show conspicuous cytoplasmic staining. | These are additional high-staining cell populations (HPA tissue IHC: High in hippocampal neuronal cells and adrenal glandular cells). Predominantly cytoplasmic signal fits the broader tissue profile (HPA tissue IHC: cytoplasmic expression in most tissues). Membrane-associated signal can also fit PRKCA localization (UniProt P17252: cell membrane). |
| Signal is confined to nuclei, or forms a uniform outline around every cell. | Treat this as discordant with the chiefly cytoplasmic tissue pattern (HPA tissue IHC: cytoplasmic expression in most tissues). Nuclear PRKCA is possible (UniProt P17252: nucleus), so nuclear staining alone does not prove an artefact; compare its cell distribution with a validated positive tissue and the run controls (general IHC practice). |
| Strong staining appears in breast adipocytes, skin fibroblasts, or smooth muscle cells. | These specific cell populations were not detected in the HPA tissue survey (HPA tissue IHC: Not detected in breast adipocytes, skin fibroblasts, and smooth muscle cells). Investigate nonspecific antibody binding or endogenous detection activity before calling the signal PRKCA (general IHC practice). HPA observations are reference patterns, not proof that every specimen will be negative. |
| The slide has diffuse haze, or a known high-staining cell population has no signal. | Diffuse haze prevents reliable compartment scoring; absent signal in Purkinje cells, hippocampal neurons, or adrenal glandular cells conflicts with the reported high staining (HPA tissue IHC: High in these cell populations). Check the positive and negative run controls before interpreting a biological difference (general IHC practice). |
💡Expected PRKCA appearanceCall a result positive when identifiable Purkinje cells, hippocampal neurons, or adrenal glandular cells show clear cytoplasmic staining, with Purkinje cell membrane staining also fitting the reference pattern (HPA tissue IHC: High in these cells; Purkinje cell cytoplasm/membrane). Uniform staining across unrelated cell types or diffuse background warrants investigation (general IHC practice).
| Cell and tissue selection | Cerebellar Purkinje cells, hippocampal neurons, and adrenal glandular cells provide high-staining reference populations; endometrial glandular cells are low, and breast adipocytes are not detected (HPA tissue IHC). Compare the named cell type, not the tissue as a whole. |
| Compartment used for scoring | The tissue profile is chiefly cytoplasmic (HPA tissue IHC). Plasma membrane localization is supported in ICC-IF, while vesicle localization is uncertain (HPA subcellular ICC-IF). UniProt also lists nucleus and mitochondrion membrane (UniProt P17252: subcellular location); those annotations do not establish that either must be visible in every paraffin section. |
| Strength of the reference pattern | HPA rates tissue IHC reliability Enhanced but reports medium consistency between staining and RNA expression (HPA tissue IHC: reliability). Four listed antibodies have Enhanced IHC validation (HPA antibodies: HPA006563, HPA006564, CAB003844, CAB016290). Use the pattern as a benchmark, while resolving discordant results with controls. |
| IF/ICC Q: Where should PRKCA appear? | A: Mainly in cytosol, with supported plasma membrane localization; vesicle localization is uncertain (HPA subcellular ICC-IF). This answers the localization question only. The ICC-IF evidence does not specify an IHC-P dilution, retrieval condition, or fluorescent staining workflow. |
| Situation | Likely cause | Next action |
|---|---|---|
| High-staining reference cells are blank. | The result conflicts with reported high signal in Purkinje cells, hippocampal neurons, or adrenal glandular cells (HPA tissue IHC). A failed staining run is one possibility (general IHC practice). | Verify tissue identity and positive-control performance; then review the validated antibody's IHC-P conditions, retrieval, detection, and reagent handling (general IHC practice). No PRKCA-specific retrieval effect is established here. |
| Signal covers the whole section as a faint haze. | Diffuse background can obscure the chiefly cytoplasmic pattern (HPA tissue IHC: tissue profile; general IHC practice). | Review the negative control, blocking, washes, antibody concentration, and chromogen development before scoring cells (general IHC practice). |
| Unexpected cells stain strongly. | Cells recorded as not detected, such as breast adipocytes or skin fibroblasts, may show nonspecific binding or endogenous detection activity (HPA tissue IHC: Not detected; general IHC practice). | Confirm the cell identity and compare primary-omission and appropriate detection controls; interpret any residual cell-specific signal cautiously (general IHC practice). |
| Only nuclei stain. | Nuclear localization is reported, but a nucleus-only section differs from the chiefly cytoplasmic tissue pattern (UniProt P17252: nucleus; HPA tissue IHC: tissue profile). | Compare a positive-control section and its cytoplasmic signal, then inspect background and detection controls before assigning nuclear PRKCA (general IHC practice). |
| All cells show identical membrane outlines. | PRKCA can associate with membrane, but the documented tissue signal varies by cell population (UniProt P17252: cell membrane; HPA tissue IHC: cell-specific levels). | Check whether cytoplasmic signal accompanies outlines in expected cells; assess negative controls and nonspecific edge staining (general IHC practice). |
| Two antibodies give different tissue patterns. | Enhanced IHC validation supports the HPA pattern but does not guarantee identical results in every specimen (HPA antibodies: Enhanced IHC; HPA tissue IHC: medium agreement with RNA). | Compare the same identified cells, compartments, and run controls across sections; report unresolved discordance rather than merging the scores (general IHC practice). |
Experiment evidence
IHC & IF images showing PRKCA staining
PB9318 · IHCIHC analysis of PKC alpha using anti-PKC alpha antibody (PB9318). PKC alpha was detected in paraffin-embedded section of mouse intestine tis
PB9318 · IHCIHC analysis of PKC alpha using anti-PKC alpha antibody (PB9318). PKC alpha was detected in paraffin-embedded section of rat intestine tissu
PA1065 · IHCIHC analysis of PKC alpha using anti-PKC alpha antibody (PA1065). PKC alpha was detected in a paraffin-embedded section of Human Mammary Can
M00743 · IHCImmunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:50 dilution.
M00743 · IHCImmunohistochemical analysis of paraffin-embedded Human thymoma, using the Antibody at 1:300 dilution.
M00743 · IFImmunofluorescent analysis using the Antibody at 1:50 dilution.
M00743-6 · IHCMouse liver was stained with anti-PKC α rabbit antibody
M00743-6 · IHCRat liver was stained with anti-PKC α rabbit antibody
A00743T495 · IHCImmunohistochemistry (IHC) analyzes of PKC-pan (T495) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
A00743T497 · IHCImmunohistochemistry (IHC) analyzes of p-PKC-pan (αT497) pAb in paraffin-embedded human breast carcinoma tissue.
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000154229-PRKCA/tissue/cerebral+cortex#imid_2007408
HPA · HippocampusHuman Protein Atlas IHC, Hippocampus. Source: https://www.proteinatlas.org/ENSG00000154229-PRKCA/tissue/hippocampal+formation#imid_2007425
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000154229-PRKCA/tissue/colon#imid_2007350
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000154229-PRKCA/tissue/liver#imid_2007343
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA006563; green = PRKCA, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA006563; green = PRKCA, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for PRKCA IHC & IF
🧪Run cerebellum first and look for PRKCA staining in Purkinje cell cytoplasm and membrane (HPA: High in Purkinje cells). Use parathyroid glandular cells as the negative tissue (HPA: Not detected); on the cerebellar slide, cells without staining provide a local background comparison, but their absence of signal does not establish that they lack PRKCA (HPA: Purkinje cell staining).
Positive control tissue: Adrenal gland (Glandular cells, HPA High)
Negative control tissue: Breast (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show PRKCA in A-431, U-251MG, U2OS, KOLF2.1J, with annotated localisation: Cytosol (supported) (HPA subcellular).
Technical controls: Include a no-primary, secondary-only control, host- and format-matched nonimmune rabbit IgG, and a PRKCA knockout sample where available; peptide blocking is another specificity check if the antibody’s immunizing peptide is available (PB9318 IHC caption: rabbit primary antibody). Quench endogenous peroxidase for DAB detection and check endogenous biotin background when using the caption’s avidin–biotin detection method (PB9318 IHC caption: SABC with DAB).
⚠️Feasibility: A target-specific fixation window or fixation effect is unreported, and the selected PB9318 paraffin-section caption does not state the fixative (PB9318 IHC caption). That caption uses heat retrieval in citrate at pH 6 for 20 minutes, but it does not establish whether retrieval is essential or whether frozen sections or IF are easier (PB9318 IHC caption). For cerebellum, confirm that the section contains Purkinje cells before interpreting absent staining (HPA: High in Purkinje cells).
HPA tissue IHC evidence for PRKCA
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Enhanced — Medium consistency between antibody staining and RNA expression data.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Glandular cells | High | Protein (IHC) | HPA → |
| Cerebellum | Purkinje cells - cytoplasm/membrane | High | Protein (IHC) | HPA → |
| Hippocampus | Neuronal cells | High | Protein (IHC) | HPA → |
| Appendix | Lymphoid tissue | Medium | Protein (IHC) | HPA → |
| Bronchus | Respiratory epithelial cells | Medium | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Breast | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Parathyroid gland | Glandular cells | Not detected | Protein (IHC) | HPA → |
| Salivary gland | Glandular cells | Not detected | Protein (IHC) | HPA → |
| Skin | Fibroblasts | Not detected | Protein (IHC) | HPA → |
| Smooth muscle | Smooth muscle cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced PRKCA IHC Tips
Troubleshoot PRKCA staining in paraffin sections by checking retrieval, cell type and compartment before comparing chromogenic signal across specimens.
What retrieval should I use when PRKCA staining is weak in paraffin sections?
Start with heat mediated retrieval in citrate buffer at pH 6 (datasheet PB9318). The selected paraffin section image used 20 minutes of retrieval before overnight incubation with 1 μg/mL primary antibody at 4°C (datasheet PB9318). If staining remains weak, compare a carefully controlled alternative retrieval condition on adjacent sections while keeping antibody concentration and detection constant (standard IHC practice). Include a section with expected PRKCA staining, such as cerebellar Purkinje cells, and judge signal against the same run’s negative control (HPA: High in cerebellar Purkinje cells; standard IHC practice).
Could fixation explain weak or uneven PRKCA staining?
Target specific fixation sensitivity is unknown because the selected paraffin section caption does not state a fixative (datasheet PB9318). Record the fixative, fixation duration, tissue thickness and processing history for each specimen before attributing differences to PRKCA biology (standard IHC practice). Compare sections processed together and hold citrate retrieval at pH 6 and antibody incubation constant while investigating a fixation variable (datasheet PB9318; standard IHC practice). If signal varies across a section, examine morphology and staining depth, then repeat on a well preserved section with the same positive and negative controls (standard IHC practice).
Which PRKCA staining compartments should I expect in chromogenic IHC?
Expect predominantly cytoplasmic signal when surveying tissue sections, while checking for cell membrane staining in appropriate cells (HPA: cytoplasmic expression in most tissues; UniProt P17252 subcellular location). HPA reports high cytoplasmic or membrane staining in cerebellar Purkinje cells, which provides a useful compartment reference (HPA: High in cerebellar Purkinje cells). Nuclear signal is biologically plausible because PRKCA can move into the nucleus during macrophage differentiation, but its presence alone does not establish that process in a section (UniProt P17252 function). Compare compartments within identified cells and inspect the no primary control before calling isolated nuclear or peripheral deposits specific (standard IHC practice).
How can I assess whether staining reflects PRKCA rather than another kinase?
Check the catalog antibody’s immunogen or mapped epitope and compare its sequence with related protein kinase C proteins before interpreting a shared staining pattern as PRKCA specific (standard antibody validation practice). The record lists 0 annotated isoforms, which does not establish antibody specificity (UniProt P17252 isoforms). PRKCA contains a C2 domain at residues 158–275 and a kinase domain at 339–597; epitope position can therefore guide a sequence comparison (UniProt P17252 domains). Use an independently validated antibody or a suitable target perturbation, where available, and require agreement in cell type and compartment (standard IHC validation practice).
How should I check PRKCA localisation by IF alongside this IHC result?
Use the separate IF/ICC guide for an IF workflow, and compare its cell level pattern with this page’s chromogenic paraffin section result (datasheet PB9318; HPA subcellular). In a relevant specimen, multiplex PRKCA with a validated marker of the expected cell type, such as a Purkinje cell marker when examining cerebellum (HPA: High in cerebellar Purkinje cells; standard IF practice). Select fluorophores after checking tissue autofluorescence and include single stain controls to assess spectral overlap (standard IF practice). PRKCA is mainly cytosolic, with supported plasma membrane localisation; if the antibody epitope is intracellular, use controlled permeabilisation and verify that membrane boundaries remain interpretable (HPA subcellular; standard IF practice).
How do I distinguish diffuse PRKCA staining from chromogenic background?
Compare the stained section with a matched no primary control, especially where diffuse DAB obscures cell borders (standard IHC practice). The selected image used 10% goat serum blocking, a biotinylated secondary antibody and streptavidin biotin detection with DAB; evaluate background from that detection sequence in the same tissue (datasheet PB9318). Include an appropriate peroxidase block and inspect the detection only control when endogenous enzyme activity or reagent binding is suspected (standard chromogenic IHC practice). If background persists, titrate primary antibody around the caption’s 1 μg/mL condition and compare specific cellular signal with adjacent unstained structures (datasheet PB9318; standard IHC practice).
What should I quantify when comparing PRKCA IHC across specimens? ⚠ ANSWER MARKED FOR VERIFICATION
Define the relevant cell population and compartment before scoring, since PRKCA can appear in cytoplasm, at membranes and in nuclei (UniProt P17252 subcellular location). For cell based staining, report percent positive cells and an H score using intensity grades 0–3, yielding a score from 0–300 (standard IHC scoring practice). For spatially restricted positive cells, report their density per mm² of viable sampled tissue (standard IHC quantification practice). Normalise counts to the number of evaluable cells or measured viable area, and apply one threshold and staining run control across compared specimens (standard IHC quantification practice).
When should I doubt an apparent PRKCA positive result?
Check whether signal lies in an expected cell and compartment: HPA reports high staining in adrenal glandular cells and cerebellar Purkinje cells, with predominantly cytoplasmic tissue expression (HPA tissue IHC). Membrane and occasional nuclear localisation can be plausible, whereas exclusively extracellular deposits warrant closer inspection because PRKCA has no transmembrane segment or signal peptide (UniProt P17252 topology and processing; UniProt P17252 subcellular location). Discount staining confined to cut edges, damaged or necrotic regions when evaluating specific cellular signal (standard IHC practice). Compare suspect areas with a no primary control and an appropriate peroxidase blocked control before attributing DAB deposits to PRKCA (standard chromogenic IHC practice).
Boster reagents
Best PRKCA / Protein kinase C alpha type IHC Antibodies
Anti-PRKCA antibodies have paraffin-section IHC images from human and mouse samples (catalog IHC captions) and an IF image for M00743 (catalog IF caption). Listed reactivity includes human, mouse and rat (catalog reactivity).
Real IHC data
Anti-PKC alpha/PRKCA Antibody ®
Cat # PB9318
Real IHC data
Anti-PKC alpha/PRKCA Antibody ®
Cat # PA1065
Real IHC data
Anti-PKC alpha PRKCA Rabbit Monoclonal Antibody
Cat # M00743
Real IHC data
Anti-PKC α Rabbit Monoclonal Antibody
Cat # M00743-6
Real IHC data
Anti-PKC-pan (T495) PRKCA Antibody
Cat # A00743T495
Real IHC data
Anti-PKC-pan ( Alpha T497) PRKCA Antibody
Cat # A00743T497
PB9318 shows mouse intestine IHC (PB9318 IHC caption); PA1065 shows human mammary cancer IHC (PA1065 IHC caption); M00743 shows human pituitary tumor IHC (M00743 IHC caption). M00743-6 shows mouse liver IHC (M00743-6 IHC caption); A00743T495 shows PKC-pan T495 in human breast carcinoma IHC (A00743T495 IHC caption); A00743T497 shows p-PKC-pan αT497 in human breast carcinoma IHC (A00743T497 IHC caption).
Which to pick: For tissue IHC, PB9318 has a paraffin-section mouse intestine example using citrate retrieval at pH 6 (PB9318 IHC caption), while PA1065 has a paraffin-section human mammary cancer example using EDTA retrieval at pH 8 (PA1065 IHC caption); the fixative is unreported in both captions (PB9318 and PA1065 IHC captions). For IF/ICC, M00743 is a rabbit monoclonal listed for both applications, with an IF image and a listed IF dilution of 1:50 (M00743 catalog entry and IF caption). For cross-species IHC, M00743-6 is a rabbit monoclonal with IHC images from human, mouse and rat samples (M00743-6 catalog entry and IHC captions).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry P17252 (KPCA_HUMAN, Protein kinase C alpha type).
- Human Protein Atlas. PRKCA tissue IHC expression (reliability: Enhanced).
- Human Protein Atlas. PRKCA subcellular location (ICC-IF): Mainly localized to the cytosol. In addition localized to the plasma membrane and vesicles..
- Human Protein Atlas. PRKCA antibody validation summary (4 antibodies).
- tsRNA-04002 alleviates intervertebral disk degeneration by targeting PRKCA to inhibit apoptosis of nucleus pulposus cells. Journal of orthopaedic surgery and research 2023 — PMC10249188.
- Clinical significance of the correlation between PLCE 1 and PRKCA in esophageal inflammation and esophageal carcinoma. Oncotarget 2017 — PMC5464868.
- Network Analysis and Experimental Verification of the Mechanisms of Hydroxysafflor Yellow A in Ischemic Stroke Following Atherosclerosis. Molecules (Basel, Switzerland) 2023 — PMC10707860.
- Protein kinase Cα suppresses Kras-mediated lung tumor formation through activation of a p38 MAPK-TGFβ signaling axis. Oncogene 2014 — PMC3895109.
- PubMed PMID:2336401 — UniProt-cited evidence.
- PubMed PMID:19054851 — UniProt-cited evidence.
- PubMed PMID:16625196 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for PRKCA (Protein kinase C alpha type). Expected localisation Cytoplasmic in most tissues; Purkinje cells also show membrane staining (HPA tissue IHC). Antigen retrieval: Citrate pH 6 HIER, heat-mediated (datasheet PB9318). Validated IHC antibody: PB9318 at 0.5-1μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Adrenal gland (Glandular cells); negative Breast. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded mouse intestine tissues; fixative not specified (datasheet PB9318); 2. Fixation: Image fixative and duration unreported (datasheet PB9318); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: Citrate pH 6 , 20 min (datasheet PB9318); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet PB9318); 8. Primary antibody: Rabbit anti-PRKCA, 0.5-1μg/ml (datasheet PB9318); 9. Primary incubation: Overnight at 4 °C (datasheet PB9318). Watch for: Fixation: Keep fixation consistent across compared paraffin sections. (selected-SKU IHC image PB9318); Caveat: Antibody staining has medium consistency with RNA expression (HPA tissue IHC); Isoform / epitope: 0 isoforms annotated; no signal peptide or transmembrane segment (UniProt); Regulation: CSF1-driven macrophage differentiation shifts PRKCA to the nucleus (UniProt). Evidence: UniProt P17252, HPA tissue IHC · Enhanced, HPA ICC-IF · Mainly localized to the cytosol. In addition localized to the plasma…, Datasheet PB9318, PMC10249188, PMC10707860. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/PRKCA/immunohistochemistry-ihc-guide.