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Product Info Summary
| SKU: | PA1065 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PKC alpha/PRKCA Antibody Picoband®
SKU/Catalog Number
PA1065
BA1355 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PKC alpha/PRKCA Antibody catalog # PA1065. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC alpha/PRKCA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1065)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PRKCA, different from the related rat and mouse sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1065 is reactive to PRKCA in Human, Mouse, Rat
Observed Molecular Weight
77 kDa
Calculated molecular weight
76.8 kDa
Background of PRKCA
Protein kinase C (PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is though to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PKC-alpha regulates cardiac contractility and propensity toward heart failure.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1065 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human U87 whole cell, human HEL whole cell, rat brain tissue, mouse brain tissue
IHC: Human Mammary Cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of PKC alpha using anti-PKC alpha antibody (PA1065).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC alpha antigen affinity purified polyclonal antibody (Catalog # PA1065) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC alpha at approximately 77 kDa. The expected band size for PKC alpha is at 80 kDa.
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IHC analysis of PKC alpha using anti-PKC alpha antibody (PA1065).
PKC alpha was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PKC alpha Antibody (PA1065) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-PKC alpha/PRKCA Antibody Picoband® (PA1065)
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Customer Q&As
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6 Customer Q&As for Anti-PKC alpha/PRKCA Antibody Picoband®
Question
I have a question about product PA1065, anti-PKC alpha/PRKCA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1065 anti-PKC alpha/PRKCA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-18
Question
Do you have a BSA free version of anti-PKC alpha/PRKCA antibody PA1065 available?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PKC alpha/PRKCA antibody PA1065 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-20
Question
Does PA1065 anti-PKC alpha/PRKCA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
It shows on the product datasheet, PA1065 anti-PKC alpha/PRKCA antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-29
Question
Please see the WB image, lot number and protocol we used for ca1 field of hippocampus using anti-PKC alpha/PRKCA antibody PA1065. Please let me know if you require anything else.
K. Banerjee
Verified customer
Asked: 2017-06-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-20
Question
Will anti-PKC alpha/PRKCA antibody PA1065 work on horse IHC with cervix carcinoma erythroleukemia?
V. Carter
Verified customer
Asked: 2017-01-10
Answer
Our lab technicians have not tested anti-PKC alpha/PRKCA antibody PA1065 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PKC alpha/PRKCA antibody PA1065 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma erythroleukemia in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-10
Question
Is a blocking peptide available for product anti-PKC alpha/PRKCA antibody (PA1065)?
R. Zhao
Verified customer
Asked: 2013-06-07
Answer
We do provide the blocking peptide for product anti-PKC alpha/PRKCA antibody (PA1065). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-06-07
