Anti-PKC alpha/PRKCA Antibody

PKC alpha antibody

Boster Bio Anti-PKC alpha/PRKCA Antibody catalog # PA1065. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 8 publication(s).

Product Info Summary

SKU: PA1065
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-PKC alpha/PRKCA Antibody

View all PKC alpha Antibodies

SKU/Catalog Number



100 μg/vial




Boster Bio Anti-PKC alpha/PRKCA Antibody catalog # PA1065. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PKC alpha/PRKCA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1065)




Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.




Rabbit IgG


A synthetic peptide corresponding to a sequence at the N-terminus of human PRKCA (153-182aa MDHTEKRGRIYLKAEVADEKLHVTVRDAKN), different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.


No cross-reactivity with other proteins

Reactive Species

PA1065 is reactive to PRKCA in Human, Mouse, Rat


PA1065 is guaranteed for IHC, WB Boster Guarantee

Background of PKC alpha

Protein kinase C (PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is though to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PKC-alpha regulates cardiac contractility and propensity toward heart failure.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For PRKCA (Source:, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

Protein kinase C alpha type




protein kinase superfamily

Alternative Names

AAG6; aging-associated gene 6; EC 2.7.11; EC; MGC129901; PKC alpha; PKCA; PKC-A; PKC-alpha; PKCAMGC129900; PRKACA; PRKCA; protein kinase C alpha type; protein kinase C, alpha PRKCA AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA protein kinase C alpha protein kinase C alpha type|PKC-A|aging-associated gene 6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRKCA, check out the PRKCA Infographic

PRKCA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRKCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PA1065 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Different effects of statins on induction of diabetes mellitus: an experimental study

Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation

5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors

Different localization and expression of protein kinase C-beta in kidney cortex of diabetic nephropathy mice and its role in telmisartan treatment

Molecular Mechanism of the Cell Death Induced by the Histone Deacetylase Pan Inhibitor LBH589 (Panobinostat) in Wilms Tumor Cells

Cheng X, Gu J, Zhang M, Yuan J, Zhao B, Jiang J, Jia X. Int Immunopharmacol. 2014 Nov;23(1):304-13. Doi: 10.1016/J.Intimp.2014.08.027. Epub 2014 Sep 16. Astragaloside Iv Inhibits Migration And Invasion In Human Lung Cancer A549 Cells Via Regulatin...

Sun D, Li X, Ma M, Liu J, Xu Y, Ye L, Hou H, Wang C, Li X, Jiang Y. Jpn J Clin Oncol. 2015 May;45(5):464-73. Doi: 10.1093/Jjco/Hyv009. Epub 2015 Feb 2. The Predictive Value And Potential Mechanisms Of Mirna-328 And Mirna-378 For Brain Metastases I...

Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...

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6 Customer Q&As for Anti-PKC alpha/PRKCA Antibody


I have a question about product PA1065, anti-PKC alpha/PRKCA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-18


We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1065 anti-PKC alpha/PRKCA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-18


Do you have a BSA free version of anti-PKC alpha/PRKCA antibody PA1065 available?

Verified Customer

Verified customer

Asked: 2019-08-20


We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PKC alpha/PRKCA antibody PA1065 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-20


Does PA1065 anti-PKC alpha/PRKCA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-05-29


It shows on the product datasheet, PA1065 anti-PKC alpha/PRKCA antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-05-29


Please see the WB image, lot number and protocol we used for ca1 field of hippocampus using anti-PKC alpha/PRKCA antibody PA1065. Please let me know if you require anything else.

K. Banerjee

Verified customer

Asked: 2017-06-20


Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-20


Will anti-PKC alpha/PRKCA antibody PA1065 work on horse IHC with cervix carcinoma erythroleukemia?

V. Carter

Verified customer

Asked: 2017-01-10


Our lab technicians have not tested anti-PKC alpha/PRKCA antibody PA1065 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PKC alpha/PRKCA antibody PA1065 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma erythroleukemia in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-01-10


Is a blocking peptide available for product anti-PKC alpha/PRKCA antibody (PA1065)?

R. Zhao

Verified customer

Asked: 2013-06-07


We do provide the blocking peptide for product anti-PKC alpha/PRKCA antibody (PA1065). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-06-07



Total: $315


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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)

$50 fee for conjugation. Antibody size is reduced to 50ug.



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