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Product Info Summary
| SKU: | A00743T495 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-PKC-pan (T495) PRKCA Antibody
SKU/Catalog Number
A00743T495
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PKC-pan (T495) PRKCA Antibody catalog # A00743T495. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC-pan (T495) PRKCA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00743T495)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 470-520 of Human PKC-pan.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00743T495 is reactive to PRKCA in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
76750 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00743T495 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of PRKCA using Anti-PKC-pan (T495) PRKCA Antibody (A00743T495).
Immunohistochemistry (IHC) analyzes of PKC-pan (T495) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-PKC-pan (T495) PRKCA Antibody A00743T495
Western blot (WB) analysis of PKC-pan (T495) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug)Lane2:COS-7 whole cell lysate(40ug)
Lane3:C6 whole cell lysate(40ug)
Lane4:MEF whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PRKCA (Source: Uniprot.org, NCBI)
Gene Name
PRKCA
Full Name
Protein kinase C alpha type
Weight
76750 MW
Superfamily
protein kinase superfamily
Alternative Names
Protein kinase C alpha type;PKC-A;PKC-alpha;2.7.11.13;PRKCA;PKCA, PRKACA; PRKCA AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA protein kinase C alpha protein kinase C alpha type|PKC-A|aging-associated gene 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCA, check out the PRKCA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKC-pan (T495) PRKCA Antibody (A00743T495)
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