THY1 Antibodies

Thy-1 membrane glycoprotein (THY1)

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. .

Gene Information

Human
Gene Name:	THY1
Uniprot:	P04216
Entrez:	7070

Family

Belongs to:
No superfamily

Alternative Names

CD90 antigen; CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen; Thy1

Molecular Weight

Mass (kDA):
17.935 kDA

Genomic Context

Human
Location:	11q23.3
Sequence:	11; NC_000011.10 (119415476..119424985, complement)

Subcellular Localizations

Cell membrane; Lipid-anchor, GPI-anchor.

Diseases

• Malignant Neoplasms
• Neoplasms
• Leukemia
• Tissue Adhesions
• Ataxia Telangiectasia
• Chromosomal Translocation
• Carcinoma
• Ovarian Neoplasm
• Malignant Neoplasm Of Ovary
• Neoplasm Metastasis
• Inflammation
• Lymphoma

• Epithelial Ovarian Cancer
• Malignant Paraganglionic Neoplasm
• Diabetes Mellitus
• Sarcoma
• Immunologic Deficiency Syndromes
• Thyroid Nodule
• Ewings Sarcoma-primitive Neuroectodermal Tumor (pnet)

Pathways

• Cell Adhesion
• Regeneration
• Spermatogenesis
• Localization
• Pathogenesis
• Dna Methylation
• Methylation
• Cell Growth
• Immune Response
• Wound Healing
• Cell Proliferation
• Innervation
• Transport

• Hyperphosphorylation
• Gene Silencing
• Programmed Cell Death
• Chemotaxis To Folate
• Fertilization
• Aging
• Hypersensitivity

PTMs

• Phosphorylation
• Methylation

• Glycosylation
• Reduction

Research Areas

• Hematopoietic Stem Cell Markers
• Innate Immunity
• Mesenchymal Stem Cell Markers
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/THY1

Boster Bio - The Best Uses Of The THY1 Marker

This article will discuss the role of Thy-1 within the hematopoietic cell stem cells. We'll discuss the regulation of Thy-1 as well as the applications for this marker on cells. We'll then discuss ways to incorporate Thy-1 into your research. Boster Bio's Anti-human CD90 THY1 Monoclonal Antibody is flow-tested and is suitable for storage of one year at 4 degC. Boster Bio has validated all of its antibodies with known positive and negative samples.

Thy-1 expression in hematopoietic cells hematopoietic cells

CD90 (Thy-1) CD90 (Thy-1) glycoprotein that is found on the cell surfaces of a variety of mammalian species. It is the marker for mesenchymal and stem cells in humans. It has several cellular functions, including regulating T cell activation, inhibiting neurite growth and acting as an adhesion molecule for cells. Thy1 is also involved in leukocyte-vascular epithelium interaction and lymphocyte development.

The Thy-1 gene is located on human chromosome 11q22.3 and mouse chromosome 9qA5.1 and covers 6.82 kb in AceView. It is located close to CD3 and CD56/NCAM genes but is not contiguous with them. Researchers believe that Thy-1 has a functional connection with CD3 delta subunit. It also maps to chromosome 11.

Transgenic mice that express Thy1 could be utilized to study MSC biology. These mice are useful to observe the recruitment of tumor-derived MSC into the matrix. Thy1-YFP mice are a great in vivo model for subcutaneous tumors. They can be helpful in observing syngeneic tumors in mice which aren't genetically altered.

The bidirectional cross-talk between Thy-1 and the other MIFs is believed to favor the growth and progression of MPN diseases. In addition, healthy and MPN-derived BM-MSC had no significant differences in morphology, proliferation, and differentiation capacity. In addition, the MIF expression of CD73-44, CD90, CD90 and CD105 was not the identical. In alpha2-cells, the bidirectional crosstalk between Thy-1, MIF of CD90 and CD73 and Sca-1 was significantly decreased.

The mice that were transgenic were injected with complete Freund's adjuvant to the pinnae. The tissue that was inflamed showed an extensive pseudo-green fluorescence. In contrast, the vascular endothelial cell population close to the tumour were highly YFP. This suggests that tumor cells could trigger Thy1 expression in hematopoietic cell lines to allow them to move. Furthermore, Thy1 on activated microvascular endothelial cells can also stimulate growth and invasion of melanoma cells.

Asthma patients are sensitive to air pollution and ozone however the underlying mechanisms aren't fully understood. In recent studies, group 2 innate lymphoid cells (ILC2) were isolated using fluorescence-activated cell sorting and immunoreactive mAbs. These cells were then depleted using anti-Thy1.2 mAbs and replaced with Thy1.1 ILC2s in mice.

Regulation of Thy-1 expression

The THY-1 marker, which is a highly conserved protein, has been thoroughly investigated for more than half a century. The molecule plays numerous functions in cell signaling. It has been found to interact with a wide number of molecules in the cell membrane and on the cell's surface. These interactions can affect signaling within the cell that has Thy-1 or the interacting cell. These interactions are still unclear.

The Thy-1 marker has a strong interaction with a variety of integrins such as syndecan 4, and a4b1, and modulates signaling pathways involved mechanotransduction. The Thy-1 marker has been implicated in the development and pathogenesis of psoriasis. This is a common chronic skin disorder that is characterized in part by the infiltration of neutrophils. The Thy-1 protein has also been found to promote migration of neutrophils into psoriasis lesion.

The Thy-1 gene is located on the mouse's chromosome 9 as a part of the immunoglobulin superfamily. It has significant homology with various species and could represent the primordial domain of immunoglobulin superfamily's ancestry. Thy1 is expressed on peripheral T cells and neurons. Thy-1 does not exist in human thymocytes.

In both normal and diseased lung tissue the Thy-1 gene is located. It is responsible for regulating the production and proliferation of collagen. It also plays a role in myofibroblast development. The expression of Thy-1 is also linked with enhanced Notch signaling. However, despite the diverse advantages of Thy-1 studies are needed to determine the most effective uses for this gene.

The Thy-1 marker can be used to identify mesenchymal stem cell lines and may also be used to monitor the adipogenic differentiation. Inflammation-induced, fibroblasts in mice that lack Thy-1 don't differentiate into adipocytes. In the same way, mesenchymal stem cell lacking Thy-1 do not transform into osteoblasts. These results confirm the role of Thy-1 in the stem cell pathway.

Boster Bio's anti-Thy-1 antibody interacts with both Human cells and Mouse cells. It can be stored at 4°C, and away from light. Boster Bio's Anti-Human CD90 Thy1 antibody has been tested against several positive and negative samples. Before making a choice, it is advisable to test the antibodies against several applications. When you're using antibodies against this protein, ensure to keep them at 4degC or higher.

Thy-1 cell marker applications

It is unclear what Thy-1 is doing as a marker for cells. It has been proven to regulate a variety of cell processes, including stemness, differentiation and survival. Further studies are necessary to understand the molecular mechanisms underlying Thy-1 function. In the meantime, ongoing studies on Thy-1 yield significant biological information. Below are some of the most important uses of Thy-1.

Thy-1 expression has been found to be highly regulated in both pathological and physiological angiogenesis. It is also elevated in the endothelium as well as blood vessels that have recently created. In rats Thy-1 expression was upregulated in adventitial blood vessels resulting from balloon injury to the carotid artery. In addition, immunoreactivity was significantly reduced after the presorption of Thy-1-Ig fusion constructs. Thy-1 is found in blood vessels of small size that have formed following the ligation of renal artery, as well as in the tunica adventitia in pregnancy.

Application of Thy-1 as a marker for cells in research has also been described. MSCs that were deficient in Thy-1 demonstrated increased differentiation capacity towards adipocytes and osteoblasts. They also expressed more osteogenic genes. These findings suggest that Thy-1 could play an important role in the differentiation and maintenance of MSCs. And, as Thy-1 is a marker for cells in the bone marrow, Thy-1 is used to identify these stem cells.

Application of Thy-1 as a marker for cells is a complex issue that has no answers. Its GPI anchor could be vital in its control. Its lipid moiety might interact with other molecules. Glycosylation may play a key role in Thy-1 effects in various contexts. It will take further research to identify its regulatory mechanisms. There is a great chance of developing new therapies in the Thy-1 manipulation and these findings could have a major impact in the near future.

Although the functions of Thy-1 aren't yet understood, it has been shown that it regulates the expression of genes in brains. It could play an important role in cell-cell and cell matrix interactions, and also in the process of regenerating nerves. It isn't known what exactly Thy-1 works however there are a number of hypotheses that support this role. A Thy-1 knockout mouse, however, is perfectly normal in all other ways except that it can learn social cues and does not respond to mazes.

In a study evaluating bone regeneration in a mouse model the Thy-1-positive periosteum-derived cells were significantly more effective than the mice in the control. These results show that Thy-1 is essential to the fate determination of MSCs. This review will look at Thy-1's functional roles on MSCs. Its influence on MSC fate decisions as well as its impact on bone and forms of adipose tissue are all covered.

There are many applications for Thy-1 as a cell marker. Thy-1 is present on a small percentage of brain cells of mice, and is also found on the fibroblasts of most vertebrate species. Thy-1 has also been proven to be a useful cell marker in tumor research. This gene is a promising candidate to detect cancerous cells as well as stem cells. While the research is ongoing , it is expected that it will help improve the management and treatment of many chronic illnesses.