Anti-THY1/Cd90 Rabbit Monoclonal Antibody

Western blot analysis of CD90 expression in human fetal brain lysate.

Characteristics of CD90 high and CD90 low gbMSCs cultured in vitro. a , b Adherent growth patterns of CD90 high and CD90 low gbMSCs cultured in 10% DMEM (×40, scale bars = 200 µm). c FACS analysis of sorted CD90 high gbMSCs ( n ≥ 3). d FACS analysis of sorted CD90 high gbMSCs ( n ≥ 3). e Growth of CD90 high and CD90 low gbMSCs cultured in 10% DMEM ( n ≥ 3). * P < 0.05, ** P < 0.01. f Wound-healing assay of CD90 high and CD90 low gbMSCs ( n ≥ 3) for 8 h with different media (×40, scale bars = 200 µm). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001
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The migration, proliferation and adhesion capacities of U87 cells incubated with different media in vitro. a Transwell assay of U87 cells cultured for 24 h in different media ( n ≥ 3) (serum-free medium, CD90 low CM and CD90 high CM). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001. Serum-free medium was used as a control. b CCK8 assay of U87 cells ( n ≥ 3) to evaluate proliferation in different media in vitro. U87 cells were incubated in 0%DMEM, CD90 high CM and CD90 low CM. * P < 0.05, ** P < 0.01, *** P < 0.001. Serum-free medium was used as a control. c Adhesion assay to estimate the effect of 0%DMEM, CD90 high CM and CD90 low CM on U87 cell adhesion. ( n ≥ 3) * P < 0.05, ** P < 0.01, *** P < 0.001. Serum-free medium were used as a control
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Tube formation capacity of gbMSCs and HUVECs incubated in different media. a Angiogenic capacity of CD90 high and CD90 low gbMSCs cultured in 0%DMEM, 10%DMEM, 0%gb-CM and S-gb-CM for 6 h on Matrigel (×100, scale bars = 100 µm). ( n ≥ 3) * P < 0.05, ** P < 0.01, *** P < 0.001. b Angiogenic capacity of HUVECs cultured in 0%DMEM, CD90 high CM and CD90 low CM for 6 h on Matrigel (×100, scale bars = 100 µm). ( n ≥ 3) * P < 0.05, ** P < 0.01. c Attachment capacity of DiO-labelled CD90 low and CD90 high gbMSCs onto vascular structures formed by HUVECs in 0%gb-CM (×100, scale bars = 100 µm). ( n ≥ 3) * P < 0.05 and ** P < 0.01
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Conditioned media from CD90 high and CD90 low gbMSCs have different functions in vivo. a Representative mice from intracranial xenograft experiments in which U87 cells with CD90 high CM (left) or U87 cells with CD90 low CM (right) were injected into the right frontal lobes of nude mice. Obviously, the sizes of the CD90 high CM group tumours were greater than those of their CD90 low CM counterparts. * P < 0.05. b Both the CD90 high CM and CD90 low CM tumour sections were stained with HE (×200, scale bars = 50 μm). In the CD90 high CM and CD90 low CM tumour tissues, IHC was employed to detect CD31 and Ki-67 expression (×400, scale bars = 25 µm). ( n ≥ 3) * P < 0.05, ** P < 0.01. c Survival curves of glioma-bearing mice. The survival times of mice implanted with U87 cells cultured with CD90 high CM were not significantly shorter than those of mice implanted with U87 cells cultured in CD90 low CM. d Double staining for CD105 (green) and CD90 (red) revealed that CD105 + CD90 − cells were located in the vessel walls, whereas CD105 + CD90 + cells were located around the tumour parenchyma. (×400, scale bars = 25 µm). e Kaplan–Meier survival curves for patients with low and high CD90 expression. The survival of glioma patients with different CD90 expression levels in TCGA database was not significantly different
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The VEGF, IL-6, bFGF, MMP9, CCL5 and SDF-1αlevels in different treatment media by ELISA. a The VEGF ( n ≥ 3) levels were significantly higher in CD90 low CM compared to those in CD90 high CM and 0%DMEM. b The bFGF ( n ≥ 3) levels were significantly higher in CD90 low CM compared to those in CD90 high CM and 0%DMEM. c The IL-6 ( n ≥ 3) levels were significantly higher in CD90 low CM compared to those in CD90 high CM and 0%DMEM. * d The SDF-1α ( n ≥ 3) levels were higher in CD90 high CM compared to those in CD90 low CM and 0%DMEM. e The CCL5 ( n ≥ 3) levels were higher in CD90 high CM compared to those in CD90 low CM and 0%DMEM. f The MMP9 ( n ≥ 3) levels were significantly higher in CD90 high CM compared to those in CD90 low CM and 0%DMEM. P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001
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Clariom D expression profiles of CD90 high and CD90 low gbMSCs ( n = 3). a Heatmap of differentially expressed lncRNAs from a microarray assay performed on CD90 high and CD90 low gbMSCs. ‘Red’ indicates high relative expression, and ‘green’ indicates low relative expression. b GO terms for the predicted targeted genes. P < 0.05 using the two-sided Fisher’s exact test was defined as statistically significant
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Immunohistochemical analysis of paraffin-embedded human kidney, using CD90 Antibody.

Immunofluorescent analysis of U87-MG cells, using CD90 Antibody.