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- Table of Contents
Thy1 antibody (A01818) performs well in IF, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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IF analysis of CD90/Thy1 using anti-CD90/Thy1 antibody (A01818).
CD90/Thy1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD90/Thy1 Antibody (A01818) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CD90/Thy1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD90/Thy1 Antibody (A01818) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-CD90/Thy1 Antibody Picoband®
Thy1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | IF, IHC, WB |
Tested Applications
Thy1 antibody was positively detected by WB in:
rat brain tissue, rai thymus tissue, mouse brain tissue, mouse thymus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Thy1 antibody was positively detected by IHC in:
mouse spleen tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Thy1 antibody was positively detected by IF in:
rat brain tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse, Rat
Immunofluorescence
5 μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Tested
Mouse
Tested
Tested
Recommend
Product Information
Product Name
Anti-CD90/Thy1 Antibody Picoband®
View all THY1 Antibodies
View all THY1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated Molecular Weight
18.1 kDa
Background of Thy1
CD90 (Cluster of Differentiation 90) or Thy-1 is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. The CD90 gene is mapped to 11q23.3. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor.
Cite This Product
Anti-CD90/Thy1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01818)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
81%
Moderate Homology
Rabbit
70%
Moderate Homology
Dog
64%
Low homology
Human
63%
Low homology
Monkey
63%
Low homology
Cow
63%
Low homology
Sheep
62%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Thy1 antibody
Immunohistochemistry (IHC) protocol for Thy1 antibody
Immunofluorescence (IF) protocol for Thy1 antibody
Publications
Application:
Species:
De Novo Transcriptome Analysis of Primed Canine Umbilical Cord Derived Mesenchymal Stem Cells Uncovers a Collection of Antimicrobial Peptides
Published: 2026-02-17
Journal: International Journal of Peptide Research and Therapeutics
Application: IF
Impact Factor: 2.40
Species: Canine
Exploring miR-21 as a key regulator in two distinct approaches of bone marrow stromal cells differentiation into Schwann-like cells
Published: 2024-05-23
Journal: SYNAPSE
Application: IF
Impact Factor: 1.60
Species: Rat
PMID: 38779935
Canine umbilical cord tissue derived mesenchymal stem cells naturally express mRNAs of some antimicrobial peptides
Published: 2023-04-15
Journal: VETERINARY RESEARCH COMMUNICATIONS
Application: IF
Impact Factor: 2.20
Species: Dog
PMID: 37059874
MiR-31 improves spinal cord injury in mice by promoting the migration of bone marrow mesenchymal stem cells
Published: 2022-09-06
Journal: PLoS One
Application: ICC
Impact Factor: 3.75
Species: Mouse
PMID: 36067193
Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes
Published: 2022-06-07
Journal: Frontiers in Bioengineering and Biotechnology
Application: FC
Impact Factor: 6.06
Species: Rat
PMID: 35747494
Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery
Published: 2020-09-11
Journal: ADVANCED FUNCTIONAL MATERIALS
Application: WB
Impact Factor: 16.84
Species: Rat
Identification and differentiation therapy strategy of pterygium in vitro
Published: 2018-08-15
Journal: American Journal of Translational Research
Application: IF
Impact Factor: 3.06
Species: Mouse
PMID: 30210698
The impact of bone marrow-derived mesenchymal stem cells on neovascularisation in rats with brain injury
Published: 2018-06-28
Journal: FOLIA NEUROPATHOLOGICA
Application: FC
Impact Factor: 1.35
Species: Rat
PMID: 30509031
Co-cultured the MSCs and cardiomyocytes can promote the growth of cardiomyocytes
Published: 2018-01-25
Journal: CYTOTECHNOLOGY
Application: IF
Impact Factor: 1.46
Species: Rat
PMID: 29372466
Therapeutic efficacy of neural stem cells originating from umbilical cord-derived mesenchymal stem cells in diabetic retinopathy.
Published: 2017-03-24
Journal: Scientific Reports
Application: IHC
Impact Factor: 4.26
Species: Rat
PMID: 28341839
Effect of Fluid Shear Stress on Cardiomyogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells
Published: 2010-12-15
Journal: ARCHIVES OF MEDICAL RESEARCH
Impact Factor: 1.88
Species: Rat
PMID: 21167388
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Product Promise
We promise Anti-CD90/Thy1 Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01818
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026