Anti-CD90/Thy1 Antibody Picoband™

Boster Bio Anti-CD90/Thy1 Antibody Picoband™ catalog # A01818. Tested in Flow Cytometry, IHC-P, WB applications. This antibody reacts with Mouse, Rat. Cited in 7 publication(s).

Product Info Summary

SKU: A01818
Size: 100μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC-P, WB

Product Name

Anti-CD90/Thy1 Antibody Picoband™

See all Thy1 products

SKU/Catalog Number

A01818

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD90/Thy1 Antibody Picoband™ catalog # A01818. Tested in Flow Cytometry, IHC-P, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD90/Thy1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01818)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A01818 is reactive to Thy1 in Mouse, Rat

Applications

A01818 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For Thy1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P01831

Gene ID

21838

Gene Name

Thy1

Full Name

Thy-1 membrane glycoprotein

Weight

18.08kDa

Alternative Names

CD90 antigen; CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen; Thy1 Thy1|CD90, T25, Thy-1, Thy-1.2.1, Thy1.2, Thy1|thymus cell antigen 1, theta|thy-1 membrane glycoprotein|thy-1 antigen

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Thy1, check out the Thy1 Infographic

Thy1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Thy1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A01818 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery
Species: Rat

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
A01818 usage in article: APP:WB, SAMPLE:MSC, DILUTION:NA

Therapeutic efficacy of neural stem cells originating from umbilical cord-derived mesenchymal stem cells in diabetic retinopathy

Differentiation of mesenchymal stem cell in the microenviroment of retinitis pigmentosa

Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.

Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells

Li F, Xu Y, Xu X, Xu B, Zhao J, Zhang X. Mol Med Rep. 2014 Jul;10(1):322-8. Doi: 10.3892/Mmr.2014.2220. Epub 2014 May 8. Fms-Related Tyrosine Kinase??3 Ligand Promotes Proliferation Of Placenta Amnion And Chorion Mesenchymal Stem Cells In??vitro.

Have you used Anti-CD90/Thy1 Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-CD90/Thy1 Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-CD90/Thy1 Antibody Picoband™

Question

We are currently using anti-CD90/Thy1 antibody A01818 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

E. Singh

Verified customer

Asked: 2020-01-28

Answer

The anti-CD90/Thy1 antibody (A01818) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-28

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD90/Thy1 antibody A01818. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-20

Question

Is this A01818 anti-CD90/Thy1 antibody reactive to the isotypes of THY1?

J. Jha

Verified customer

Asked: 2019-08-26

Answer

The immunogen of A01818 anti-CD90/Thy1 antibody is E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-26

Question

Will anti-CD90/Thy1 antibody A01818 work for ICC with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

According to the expression profile of leukemic t-cell, THY1 is highly expressed in leukemic t-cell. So, it is likely that anti-CD90/Thy1 antibody A01818 will work for ICC with leukemic t-cell.

Boster Scientific Support

Answered: 2019-08-21

Question

I am interested in using your anti-CD90/Thy1 antibody for positive regulation of release of sequestered calcium ion into cytosol studies. Has this antibody been tested with western blotting on lung tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

Thank you for your inquiry. This A01818 anti-CD90/Thy1 antibody is validated on mouse thymus tissue, brain tissue, lung tissue, rat thymus tissue. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-21

Question

Is a blocking peptide available for product anti-CD90/Thy1 antibody (A01818)?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

We do provide the blocking peptide for product anti-CD90/Thy1 antibody (A01818). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-09

Question

We have observed staining in rat leukemic t-cell. Do you have any suggestions? Is anti-CD90/Thy1 antibody supposed to stain leukemic t-cell positively?

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

From literature leukemic t-cell does express THY1. From Uniprot.org, THY1 is expressed in anterior cingulate cortex, brain, amygdala, eye, liver, leukemic t-cell, among other tissues. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2019-08-01

Question

We bought anti-CD90/Thy1 antibody for Flow Cytometry on leukemic t-cell in a previous project. I am using mouse, and We are going to use the antibody for IHC next. I am looking for examining leukemic t-cell as well as amygdala in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

I looked at the website and datasheets of our anti-CD90/Thy1 antibody and it seems that A01818 has been tested on mouse in both Flow Cytometry and IHC. Thus A01818 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-06-17

Question

I see that the anti-CD90/Thy1 antibody A01818 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-29

Question

I was wanting to use your anti-CD90/Thy1 antibody for ICC for rat leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukemic t-cell identification?

Verified Customer

Verified customer

Asked: 2019-02-04

Answer

As indicated on the product datasheet, A01818 anti-CD90/Thy1 antibody has been validated for Flow Cytometry, IHC, ICC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-04

Question

Can you help my question with product A01818, anti-CD90/Thy1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-09-21

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01818 anti-CD90/Thy1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-09-21

Question

Will A01818 anti-CD90/Thy1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-04-12

Answer

As indicated on the product datasheet, A01818 anti-CD90/Thy1 antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-04-12

Question

We are interested in to test anti-CD90/Thy1 antibody A01818 on rat leukemic t-cell for research purposes, then I may be interested in using anti-CD90/Thy1 antibody A01818 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-03-02

Answer

The products we sell, including anti-CD90/Thy1 antibody A01818, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-03-02

Question

Our lab were satisfied with the WB result of your anti-CD90/Thy1 antibody. However we have seen positive staining in amygdala cell membrane using this antibody. Is that expected? Could you tell me where is THY1 supposed to be expressed?

K. Miller

Verified customer

Asked: 2015-05-06

Answer

According to literature, amygdala does express THY1. Generally THY1 expresses in cell membrane. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2015-05-06

Question

Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-CD90/Thy1 antibody A01818. Please let me know if you require anything else.

L. Jha

Verified customer

Asked: 2014-08-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-08-22

Question

Is there a BSA free version of anti-CD90/Thy1 antibody A01818 available?

Z. Baker

Verified customer

Asked: 2013-10-15

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD90/Thy1 antibody A01818 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-10-15

Conjugation

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
A01818
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.