Click image to see more details
Product Info Summary
| SKU: | A01818-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD90/Thy1 Antibody Picoband®
SKU/Catalog Number
A01818-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD90/Thy1 Antibody Picoband® catalog # A01818-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD90/Thy1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01818-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD90/Thy1 recombinant protein (Position: Q20-H111).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01818-1 is reactive to THY1 in Human, Mouse, Rat
Observed Molecular Weight
22 kDa
Calculated molecular weight
17.9 kDa
Background of THY1
CD90 (Cluster of Differentiation 90) or Thy-1 is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. The CD90 gene is mapped to 11q23.3. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01818-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human U251 whole cell, rat brain tissue, rat thymus tissue, mouse brain tissue
IHC: human brain tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CD90/Thy1 using anti-CD90/Thy1 antibody (A01818-1).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD90/Thy1 antigen affinity purified polyclonal antibody (Catalog # A01818-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 22 kDa. The expected band size for CD90/Thy1 is at 18 kDa.
Click image to see more details
IHC analysis of CD90/Thy1 using anti-CD90/Thy1 antibody (A01818-1).
CD90/Thy1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD90/Thy1 Antibody (A01818-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-CD90/Thy1 Antibody Picoband® (A01818-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD90/Thy1 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CD90/Thy1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-CD90/Thy1 Antibody Picoband®
Question
We were well pleased with the WB result of your anti-CD90/Thy1 antibody. However we have seen positive staining in amygdala cell membrane using this antibody. Is that expected? Could you tell me where is THY1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
From literature, amygdala does express THY1. Generally THY1 expresses in cell membrane. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2020-02-04
Question
We are currently using anti-CD90/Thy1 antibody A01818-1 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
The anti-CD90/Thy1 antibody (A01818-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-02
Question
We have been able to see staining in human eye. Are there any suggestions? Is anti-CD90/Thy1 antibody supposed to stain eye positively?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
According to literature eye does express THY1. According to Uniprot.org, THY1 is expressed in anterior cingulate cortex, brain, amygdala, eye, liver, leukemic t-cell, among other tissues. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2018-12-14
Question
I would like using your anti-CD90/Thy1 antibody for integrin-mediated signaling pathway studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-02-28
Answer
Thank you for your inquiry. This A01818-1 anti-CD90/Thy1 antibody is validated on rat thymus tissue, brain tissue, mouse brain. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-02-28
Question
We bought anti-CD90/Thy1 antibody for WB on brain in a previous project. I am using human, and We want to use the antibody for ELISA next. I am interested in examining brain as well as amygdala in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
S. Baker
Verified customer
Asked: 2018-01-03
Answer
I viewed the website and datasheets of our anti-CD90/Thy1 antibody and I see that A01818-1 has been tested on human in both WB and ELISA. Thus A01818-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-01-03
