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Product Info Summary
| SKU: | M01141 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® (monoclonal, 6G5)
SKU/Catalog Number
M01141
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® (monoclonal, 6G5) catalog # M01141. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® (monoclonal, 6G5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01141)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
6G5
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 zeta/delta, which shares 97.8% amino acid (aa) sequence identity with both mouse and rat 14-3-3 zeta/delta.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01141 is reactive to YWHAZ in Human, Monkey, Mouse, Rat
Observed Molecular Weight
28 kDa
Calculated molecular weight
27.7 kDa
Background of YWHAZ
14-3-3 protein zeta/delta (14-3-3ζ) is a protein that in humans is encoded by the YWHAZ gene on chromosome 8. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01141 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Monkey, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell,, human A549 whole cell,, monkey COS-7 whole cell,, human Raji whole cell,, PC-3 cell, SiHa cell
Validation Images & Assay Conditions
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Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates;
Lane 2: human A549 whole cell lysates;
Lane 3: monkey COS-7 whole cell lysates;
Lane 4: human Raji whole cell lysates;
Lane 5:huamn Caco-2 whole cell lysates;
Lane 6: huamn Jurkat whole cell lysates;
Lane 7: mouse brain tissue lysates;
Lane 8: rat brain tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-14-3-3 zeta/delta antigen affinity purified monoclonal antibody (Catalog # M01141) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.
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Flow Cytometry analysis of PC-3 cells using anti-14-3-3 zeta/delta antibody (M01141).
Overlay histogram showing PC-3 cells stained with M01141 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-14-3-3 zeta/delta Antibody (M01141,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Flow Cytometry analysis of SiHa cells using anti-14-3-3 zeta/delta antibody (M01141).
Overlay histogram showing SiHa cells stained with M01141 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-14-3-3 zeta/delta Antibody (M01141,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® (monoclonal, 6G5) (M01141)
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