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Product Info Summary
| SKU: | M01141-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-YWHAZ Antibody
SKU/Catalog Number
M01141-2
Size
80 µl
Description
Boster Bio Anti-YWHAZ Antibody (Catalog # M01141-2). Tested in IF, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-YWHAZ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01141-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1314CT423.108.153.173.140
Isotype
IgG2b,κ
Immunogen
This YWHAZ antibody is generated from a mouse immunized with a recombinant protein from human YWHAZ.
Reactive Species
M01141-2 is reactive to YWHAZ in Human, Mouse, Rat
Calculated molecular weight
27.7 kDa
Background of YWHAZ
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01141-2 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
Validation Images & Assay Conditions
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Fluorescent image of U251 cells stained with YWHAZ Antibody. M01141-2 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Western blot analysis of lysates from A431, HepG2, mouse NIH/3T3, rat PC-12 and C6 cell line, mouse brain tissue (from left to right), using YWHAZ Antibody . M01141-2 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
Specific Publications For Anti-YWHAZ Antibody (M01141-2)
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