Anti-Histone H3 (acetyl K9) Rabbit Monoclonal Antibody

Western blot analysis of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody (M12477-25).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human T98G whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human SH-SY5Y whole cell lysates,
Lane 7: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 (acetyl K9) antigen affinity purified monoclonal antibody (M12477-25) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Histone H3 (acetyl K9) at approximately 15 kDa. The expected band size for Histone H3 (acetyl K9) is at 15 kDa.

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Western blot analysis of Histone H3 (acetyl K9) expression in C6 cell lysate treated with Trichostatin A.

IHC analysis of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody (M12477-25).
Histone H3 (acetyl K9) was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H3 (acetyl K9) Antibody (M12477-25) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody (M12477-25).
Histone H3 (acetyl K9) was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H3 (acetyl K9) Antibody (M12477-25) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody (M12477-25).
Histone H3 (acetyl K9) was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H3 (acetyl K9) Antibody (M12477-25) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody (M12477-25).
Histone H3 (acetyl K9) was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H3 (acetyl K9) Antibody (M12477-25) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody (M12477-25).
Histone H3 (acetyl K9) was detected in a paraffin-embedded section of human breast tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H3 (acetyl K9) Antibody (M12477-25) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.