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Product Info Summary
| SKU: | AZB0S602 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish FN1A Antibody Picoband®
SKU/Catalog Number
AZB0S602
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish FN1A Antibody Picoband® catalog # AZB0S602. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish FN1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZB0S602)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish FN1A recombinant protein (Position: F1936-R2241).
Reactive Species
AZB0S602 is reactive to FN1A in Zebrafish
Background of FN1A
Enables integrin binding activity. Acts upstream of or within with a positive effect on pronephric nephron tubule morphogenesis. Acts upstream of or within several processes, including chordate embryonic development; circulatory system development; and epithelial cell differentiation. Located in interstitial matrix. Is expressed in several structures, including cardiovascular system; mesoderm; nervous system; tail bud; and yolk. Human ortholog(s) of this gene implicated in calcium oxalate nephrolithiasis; membranoproliferative glomerulonephritis; and spondylometaphyseal dysplasia corner fracture type. Orthologous to human FN1 (fibronectin 1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZB0S602 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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Western blot analysis of FN1A using anti-FN1A antibody (AZB0S602).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: whole female zebrafish tissue lysates,
Lane 3: whole male zebrafish tissue lysates,
Lane 4: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FN1A antigen affinity purified polyclonal antibody (AZB0S602) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FN1A at approximately 271 kDa. The expected band size for FN1A is at 271 kDa.
Specific Publications For Anti-Zebrafish FN1A Antibody Picoband® (AZB0S602)
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