Product Info Summary
SKU: | LS001457 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2 |
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Product info
Product Name
CKII alpha (CSNK2A1) (NM_001895) Human Over-expression Lysate
See all Casein Kinase 2 alpha products
SKU/Catalog Number
LS001457
Size
100 µg
Description
Transient overexpression lysate of casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2
Storage & Handling
Cite This Product
CKII alpha (CSNK2A1) (NM_001895) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001457)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
![ls001457 ckii alpha csnk2a1 human over expression lysates nm 001895 wb testing 1 ls001457 ckii alpha csnk2a1 human over expression lysates nm 001895 wb testing 1](https://www.bosterbio.com/media/catalog/product/l/s/ls001457-ckii-alpha-csnk2a1-human-over-expression-lysates-nm-001895-wb-testing-1.jpg)
Click image to see more details
CKII alpha (CSNK2A1) Human Over-expression Lysates NM_001895.
Western blot validation of overexpression lysate (LS001457) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CSNK2A1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CSNK2A1 (Source: Uniprot.org, NCBI)
Gene Name
CSNK2A1
Full Name
Casein kinase II subunit alpha
Weight
45.144kDa
Superfamily
protein kinase superfamily
Alternative Names
Casein Kinase 2 alpha; casein kinase 2, alpha 1 polypeptide; casein kinase II alpha 1 subunit; CK II alpha; CK2 catalytic subunit alpha; CK2A1; CK2A1casein kinase II subunit alpha; CKII; CSNK2A1; EC 2.7.11; EC 2.7.11.1; protein kinase CK2 CSNK2A1 CK2A1, CKII, Cka1, Cka2, OCNDS casein kinase 2 alpha 1 casein kinase II subunit alpha|CK2 catalytic subunit alpha|casein kinase 2, alpha 1 polypeptide|casein kinase II alpha 1 polypeptide pseudogene|casein kinase II alpha 1 subunit|protein kinase CK2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CSNK2A1, check out the CSNK2A1 Infographic
![CSNK2A1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSNK2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CKII alpha (CSNK2A1) (NM_001895) Human Over-expression Lysate (LS001457)
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