GRWD1 (NM_031485) Human Over-expression Lysate

Lysate of GRWD1 over-expression cell lines, WB control

Transient overexpression lysate of glutamate-rich WD repeat containing 1 (GRWD1)

Product Info Summary

SKU: LS083743
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of glutamate-rich WD repeat containing 1 (GRWD1)

Product Name

GRWD1 (NM_031485) Human Over-expression Lysate

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SKU/Catalog Number

LS083743

Size

100 µg

Description

Transient overexpression lysate of glutamate-rich WD repeat containing 1 (GRWD1)

Storage & Handling

Cite This Product

GRWD1 (NM_031485) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083743)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for GRWD1 (Source: Uniprot.org, NCBI)

Uniprot ID

Q9BQ67

Gene ID

83743

Gene Name

GRWD1

Full Name

Glutamate-rich WD repeat-containing protein 1

Weight

49.419kDa

Alternative Names

CDW4; CUL4- and DDB1-associated WDR protein 4; glutamate-rich WD repeat containing 1; GRWDglutamate rich WD repeat protein GRWD; KIAA1942regulator of ribosome biogenesis 1 homolog; RRB1; WD repeat domain 28; WDR28glutamate-rich WD repeat-containing protein 1 GRWD1 CDW4, GRWD, RRB1, WDR28 glutamate rich WD repeat containing 1 glutamate-rich WD repeat-containing protein 1|CUL4- and DDB1-associated WDR protein 4|WD repeat domain 28|glutamate rich WD repeat protein GRWD|regulator of ribosome biogenesis 1 homolog

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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