Click image to see more details
Product Info Summary
|Description:||Transient overexpression lysate of glutamate-rich WD repeat containing 1 (GRWD1)|
GRWD1 (NM_031485) Human Over-expression Lysate
Transient overexpression lysate of glutamate-rich WD repeat containing 1 (GRWD1)
Storage & Handling
Cite This Product
GRWD1 (NM_031485) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083743)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
Click image to see more details
GRWD1 Human Over-expression Lysates NM_031485.
Western blot validation of overexpression lysate (LS083743) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with GRWD1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for GRWD1 (Source: Uniprot.org, NCBI)
Glutamate-rich WD repeat-containing protein 1
CDW4; CUL4- and DDB1-associated WDR protein 4; glutamate-rich WD repeat containing 1; GRWDglutamate rich WD repeat protein GRWD; KIAA1942regulator of ribosome biogenesis 1 homolog; RRB1; WD repeat domain 28; WDR28glutamate-rich WD repeat-containing protein 1 GRWD1 CDW4, GRWD, RRB1, WDR28 glutamate rich WD repeat containing 1 glutamate-rich WD repeat-containing protein 1|CUL4- and DDB1-associated WDR protein 4|WD repeat domain 28|glutamate rich WD repeat protein GRWD|regulator of ribosome biogenesis 1 homolog*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on GRWD1, check out the GRWD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRWD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for LS083743
Here are featured tools and databases that you might find useful:
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used GRWD1 (NM_031485) Human Over-expression Lysate?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review GRWD1 (NM_031485) Human Over-expression Lysate
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question