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Product Info Summary
| SKU: | FEK1933 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) and urine |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human CFHR5 PicoKine® Quick ELISA Kit
See all Complement Factor H-related 5/CFHR5 products
SKU/Catalog Number
FEK1933
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CFHR5 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CFHR5 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 0.1 ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human CFHR5 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1933)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E19-E569
Sensitivity
<0.1 ng/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
See datasheet of FEK1933 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1933 is reactive to CFHR5 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA) and urine
Application Guarantee
FEK1933 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Complement Factor H-related 5/CFHR5
Complement factor H-related protein 5 is a protein that in humans is encoded by the CFHR5 gene. It is mapped to 1q31.3. This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS).
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1933-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1933-ST | Human Complement Factor H-related 5/CFHR5 Standard | 2 | 50 ng/tube |
| FEK1933-A | Human CFHR5 Antibody A | 1 | 3ml |
| FEK1933-B | Human CFHR5 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.028 | 0.269 | 0.470 | 0.722 | 1.123 | 1.623 | 1.985 | 2.387 |
Data Example Images
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Human CFHR5 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CFHR5 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1228 | 5795 | 26941 | 1325 | 6341 | 26498 |
| Standard deviation | 81.05 | 376.68 | 1373.99 | 84.8 | 374.12 | 1563.38 |
| CV(%) | 6.6% | 6.5% | 5.1% | 6.4% | 5.9% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Complement Factor H-related 5/CFHR5 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1228 | 1345 | 1463 | 1512 | 1387 | 81.83 | 5.9% |
| Sample 2 | 5795 | 6654 | 5963 | 5794 | 6052 | 387.3 | 6.4% |
| Sample 3 | 26491 | 29746 | 25641 | 26758 | 27272 | 1472.66 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For CFHR5 (Source: Uniprot.Org, NCBI)
Gene Name
CFHR5
Full Name
Complement factor H-related protein 5
Weight
64.419kDa
Alternative Names
CFHL5; CFHL5factor H-related protein 5; CFHR5; Complement Factor Hrelated 5; Complement Factor H-related 5; FHR5; FHR-5complement factor H-related protein 5; FHR5MGC133240; FLJ10549 CFHR5 CFHL5D, FHR-5, FHR5, CFHR5 complement factor H related 5 complement factor H-related protein 5|factor H-related protein 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CFHR5, check out the CFHR5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CFHR5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CFHR5 PicoKine® Quick ELISA Kit (FEK1933)
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