Mouse CCL25/TECK PicoKine™ Quick ELISA Kit

Mouse CCL25/TECK PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse CCL25/TECK in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: FEK1424
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Assay Time: 90 Minutes

Product Name

Mouse CCL25/TECK PicoKine™ Quick ELISA Kit

See all CCL25/TECK products

SKU/Catalog Number

FEK1424

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CCL25/TECK PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse CCL25/TECK in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse CCL25/TECK PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1424)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-N144

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1424 is reactive to Ccl25 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK1424 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog numberDescription QuantityVolume
FEK1424-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK1424-STMouse CCL25/TECK Standard 2 10 ng/tube
FEK1424-CMouse Ccl25 Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)031.262.51252505001,0002,000
O.D.0.0210.1050.1610.2890.5571.0031.6852.351

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)9236494197276984
Standard deviation4.9717.4270.585.9220.766.91
CV (%)5.4%6.6%7.5%6.1%7.5%6.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL25/TECK in ELISA kits from four different production batches/lots.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 192988697933.083.3%
Sample 226428425726626818.476.9%
Sample 394198598195396527.992.9%
*number of samples for each test n=16.

Gene/Protein Information For Ccl25 (Source: Uniprot.Org, NCBI)

Uniprot ID

O35903

Gene ID

20300

Gene Name

Ccl25

Full Name

C-C motif chemokine 25

Weight

16.733kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine 25; CCL25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; Ckb15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECK; TECKMGC150327; thymus expressed chemokine; Thymus-expressed chemokine Ccl25|A130072A22Rik, AI852536, CKb15, Scya, Scya25, TECK|chemokine (C-C motif) ligand 25|C-C motif chemokine 25|small inducible chemokine 25|small inducible cytokine A25|thymus-expressed chemokine

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ccl25, check out the Ccl25 Infographic

Ccl25 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ccl25: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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