Anti-Zebrafish cdhr1a Antibody

IF analysis of Cdhr1a using anti-Cdhr1a antibody (DZ07988).
Cdhr1a was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Cdhr1a Antibody (DZ07988) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Evolutionarily conserved localization of pcdh15 and cdhr1 in photoreceptors predicts interactions linking outer segments and calyceal processes. A-A”) Confocal microscopy of wildtype 5dpf retinal cryosections probed with cdhr1a antibody (green), Peanut germ agglutinin (PNA - magenta), Wheat germ agglutinin (WG - teal) and pcdh15b (red). White boxes indicate the location of the inset enlargement. White arrowheads highlight the linear localization of cdhr1a along cone OSs (A) and pcdh15b outlining the calyceal process (A’). Merge of all three signals highlights the proximity between cdhr1a and pcdh15b (A”). Scale bar = 10μm. B) Confocal microscopy of wildtype 5dpf retinal cryosections probed with cdhr1a antibody (green), pcdh15b (red) and WGA (teal). White boxes indicate the location of the inset enlargement. White arrowheads highlight the linear localization of cdhr1a along rod OSs (B) and pcdh15b outlining the calyceal process (B’). Merge of all three signals highlights the proximity between cdhr1a and pcdh15b (B”). Scale bar = 10μm. C) Structured illumination microscopy (SIM) of wildtype zebrafish retinal cryosections probed with cdhr1a (green) and pcdh15b (red). White box represents the inset enlargement. White arrowheads highlight the juxtaposition of the cdhr1a and pcdh15b signals. Scale bar = 10μm. D) Diagrammatic model of the connection between the OS discs and CPs in both rod and cone cells mediated by the interaction between OS bound cdhr1a and CP bound pcdh15b. E-L) Structured illumination microscopy (SIM) of wildtype xenopus (E), mallard duck (F), mouse (G), rat (H), spiny mouse (I), gerbil (J), macaque (K) and human (L) retinal sections probed for cdhr1 (green) and pcdh15 (red). White boxes represent the inset enlargements. White arrowheads highlight the juxtaposition of the cdhr1a and pchd15b signals in each species. The model figure was prepared using Biorender. Scale bar = 10μm.
Index in eLife under a CC BY license. DOI: 10.7554/eLife.102258.1

IHC analysis of Cdhr1a using anti-Cdhr1a antibody (DZ07988).
Cdhr1a was detected in paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdhr1a Antibody (DZ07988) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of Cdhr1a using anti-Cdhr1a antibody (DZ07988).
Cdhr1a was detected in paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdhr1a Antibody (DZ07988) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.