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Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)

SKU MA1072
Size 100μg/vial
Reactivity Goat, Human, Pig, Rat
Clonality Monoclonal
Clone Number NOS-B1
Host Mouse
Ig Isotype Mouse IgG1
Applications WB

Overview

Product Name Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)
SKU/Catalog Number MA1072
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Nitric Oxide Synthase, Brain (1-181) NOS1, nitric oxide synthase 1 (neuronal) (NOS1) detection. Tested with WB in Human;rat;goat;pig. No cross reactivity with other proteins.
Cite This Product Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1072)
Host Mouse
Immunogen Recombinant neuronal NOS fragment(amino acids 1-181) from rat brain.
Reactivity Goat, Human, Pig, Rat

Assay Details

Assay Dilutions Overview

Western blot, 0.5μg/ml, Human, goat, pig, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P29476
Gene Name Nos1
Protein Name Nitric oxide synthase, brain
Tissue Specificity Isoform N-NOS-1 is expressed in brain and colorectum. Found in the Auerbach's plexus of the enteric nervous system. Isoform PNNOS is expressed in the penis, urethra, prostate, and skeletal muscle, and coexists with the cerebellar nnos in the pelvic plexus, bladder and liver, and is detectable in the cerebellum. .
Alternative Names Nitric oxide synthase, brain;1.14.13.39;BNOS;Constitutive NOS;NC-NOS;N
Subcellular Localization Cell membrane, sarcolemma ; Peripheral membrane protein . Cell projection, dendritic spine . In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex (By similarity). In neurons, enriched in dendritic spines. .
Molecular Weight 160559 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non- adrenergic and non-cholinergic nerves in the colorectum.
Background Nitric Oxide Synthase 1(NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is though to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal(Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.

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Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240
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Publications

Dhillon Ss, Mastropaolo La, Murchie R, Griffiths C, Th??ni C, Elkadri A, Xu W, Mack A, Walters T, Guo C, Mack D, Huynh H, Baksh S, Silverberg Ms, Brumell Jh, Snapper Sb, Muise Am. Clin Transl Gastroenterol. 2014 Jan 16;5:E46. Doi: 10.1038/Ctg.2013....
Li J, Wang Z, Cao J, Dong Yl, Chen Yx. Anat Rec (Hoboken). 2014 Jul;297(7):1331-7. Doi: 10.1002/Ar.22909. Epub 2014 Apr 4. Role Of Monochromatic Light On Development Of Cecal Tonsil In Young Broilers.
Gao S, Long Cl, Wang Rh, Wang H. Cardiovasc Res. 2009 Aug 1;83(3):444-56. Doi: 10.1093/Cvr/Cvp099. Epub 2009 Mar 20. K(Atp) Activation Prevents Progression Of Cardiac Hypertrophy To Failure Induced By Pressure Overload Via Protecting Endothelial F...
Hou Sx, Zhu Wj, Pang Mq, Jeffry J, Zhou Ll. Food Chem Toxicol. 2014 Feb;64:57-64. Doi: 10.1016/J.Fct.2013.11.022. Epub 2013 Nov 26. Protective Effect Of Iridoid Glycosides From Paederia Scandens (Lour.) Merrill (Rubiaceae) On Uric Acid Nephropathy...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to nos antibody, nnos antibody, no antibody, nos1 antibody