|Validated Species:||Goat, Human, Pig, Rat|
Data & Images
|Product Name||Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)|
|Description||Mouse IgG monoclonal antibody for Nitric Oxide Synthase, Brain (1-181) NOS1, nitric oxide synthase 1 (neuronal) (NOS1) detection. Tested with WB in Human;rat;goat;pig. No cross reactivity with other proteins.|
|Cite This Product||Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1) NOS-B1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1072)|
|Replacement Item||This antibody may replace the following items: sc-648|sc-1025|sc-55521|sc-8309|sc-17825|sc-5302|sc-136006|sc-398843 from Santa Cruz Biotechnology.|
|Validated Species||Goat, Human, Pig, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant neuronal NOS fragment(amino acids 1-181) from rat brain.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Nitric oxide synthase, brain|
|Molecular Weight||160559 MW|
|Protein Function||Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non- adrenergic and non-cholinergic nerves in the colorectum.|
|Tissue Specificity||Isoform N-NOS-1 is expressed in brain and colorectum. Found in the Auerbach's plexus of the enteric nervous system. Isoform PNNOS is expressed in the penis, urethra, prostate, and skeletal muscle, and coexists with the cerebellar nnos in the pelvic plexus, bladder and liver, and is detectable in the cerebellum. .|
|Sequence Similarities||Belongs to the NOS family.|
|Subcellular Localization||Cell membrane, sarcolemma ; Peripheral membrane protein . Cell projection, dendritic spine . In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex (By similarity). In neurons, enriched in dendritic spines. .|
|Alternative Names||Nitric oxide synthase, brain;18.104.22.168;BNOS;Constitutive NOS;NC-NOS;N|
Background for Nitric oxide synthase, brain
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.5μg/ml, Human, goat, pig, rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.