Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)

Boster Bio Anti-Nitric Oxide Synthase, Brain (1-181) NOS1 Antibody (Monoclonal, NOS-B1) catalog # MA1072. Tested in WB applications. This antibody reacts with Goat, Human, Pig, Rat. Cited in 5 publication(s).

Product Info Summary

SKU: MA1072
Size: 100μg/vial
Reactive Species: Goat, Human, Pig, Rat
Host: Mouse
Application: WB

Product Name

Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)

See all nNOS products

SKU/Catalog Number

MA1072

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Nitric Oxide Synthase, Brain (1-181) NOS1 Antibody (Monoclonal, NOS-B1) catalog # MA1072. Tested in WB applications. This antibody reacts with Goat, Human, Pig, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1072)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

NOS-B1

Isotype

Mouse IgG1

Immunogen

Recombinant neuronal NOS fragment (amino acids 1-181) from rat brain.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1072 is reactive to Nos1 in Goat, Human, Pig, Rat

Applications

MA1072 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.5μg/ml, Human, goat, pig, rat

Validation Images & Assay Conditions

Gene/Protein Information For Nos1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P29476

Gene ID

24598

Gene Name

Nos1

Full Name

Nitric oxide synthase, brain

Weight

160.559kDa

Superfamily

NOS family

Alternative Names

bNOS; brain; Constitutive NOS; EC 1.14.13.39; IHPS1; Neuronal NOS; nitric oxide synthase 1 (neuronal); nNOS; N-NOS; nNOSNOS type I; NOS; NOS1; Peptidyl-cysteine S-nitrosylase NOS1 Nos1|bNOS|nitric oxide synthase 1|nitric oxide synthase, brain|N-NOS|NC-NOS|NOS type I|constitutive NOS|nNOS|neuronal NOS|nitric oxidase synthase|nitric oxide synthase 1, neuronal|peptidyl-cysteine S-nitrosylase NOS1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Nos1, check out the Nos1 Infographic

Nos1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Nos1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1072 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Distribution of constitutive nitric oxide synthase in the jejunum of adult rat

Dhillon Ss, Mastropaolo La, Murchie R, Griffiths C, Th??ni C, Elkadri A, Xu W, Mack A, Walters T, Guo C, Mack D, Huynh H, Baksh S, Silverberg Ms, Brumell Jh, Snapper Sb, Muise Am. Clin Transl Gastroenterol. 2014 Jan 16;5:E46. Doi: 10.1038/Ctg.2013...

Li J, Wang Z, Cao J, Dong Yl, Chen Yx. Anat Rec (Hoboken). 2014 Jul;297(7):1331-7. Doi: 10.1002/Ar.22909. Epub 2014 Apr 4. Role Of Monochromatic Light On Development Of Cecal Tonsil In Young Broilers.

Gao S, Long Cl, Wang Rh, Wang H. Cardiovasc Res. 2009 Aug 1;83(3):444-56. Doi: 10.1093/Cvr/Cvp099. Epub 2009 Mar 20. K(Atp) Activation Prevents Progression Of Cardiac Hypertrophy To Failure Induced By Pressure Overload Via Protecting Endothelial F...

Hou Sx, Zhu Wj, Pang Mq, Jeffry J, Zhou Ll. Food Chem Toxicol. 2014 Feb;64:57-64. Doi: 10.1016/J.Fct.2013.11.022. Epub 2013 Nov 26. Protective Effect Of Iridoid Glycosides From Paederia Scandens (Lour.) Merrill (Rubiaceae) On Uric Acid Nephropathy...

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3 Customer Q&As for Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)

Question

We are currently using anti-Nitric Oxide Synthase, Brain(1-181) NOS1 antibody (Monoclonal, NOS-B1) MA1072 for goat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says goat, human, pig, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2017-10-09

Answer

The anti-Nitric Oxide Synthase, Brain(1-181) NOS1 antibody (Monoclonal, NOS-B1) (MA1072) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-09

Question

We were content with the WB result of your anti-Nitric Oxide Synthase, Brain(1-181) NOS1 antibody (Monoclonal, NOS-B1). However we have seen positive staining in cerebellum cell membrane using this antibody. Is that expected? Could you tell me where is NOS1 supposed to be expressed?

G. Zhang

Verified customer

Asked: 2015-04-03

Answer

According to literature, cerebellum does express NOS1. Generally NOS1 expresses in cell membrane, sarcolemma. Regarding which tissues have NOS1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7678401
Cerebellum, Pubmed ID: 7515942
Retina, Pubmed ID: 8879752
Skeletal muscle, Pubmed ID: 9791007
Testis, Pubmed ID: 9111048

Boster Scientific Support

Answered: 2015-04-03

Question

We have been able to see staining in rat skeletal muscle. Any tips? Is anti-Nitric Oxide Synthase, Brain(1-181) NOS1 antibody (Monoclonal, NOS-B1) supposed to stain skeletal muscle positively?

C. Zhao

Verified customer

Asked: 2013-08-19

Answer

According to literature skeletal muscle does express NOS1. According to Uniprot.org, NOS1 is expressed in vastus lateralis, cerebellum, brain, retina, testis, skeletal muscle, among other tissues. Regarding which tissues have NOS1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7678401
Cerebellum, Pubmed ID: 7515942
Retina, Pubmed ID: 8879752
Skeletal muscle, Pubmed ID: 9791007
Testis, Pubmed ID: 9111048

Boster Scientific Support

Answered: 2013-08-19

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