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CLIPB4 antibody (DZ33989-1) performs well in WB and shows reactivity with African malaria mosquito.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of CLIPB4 using anti-CLIPB4 antibody (DZ33989-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mosquito hemolymph β-galactosidase KD lysates,
Lane 2: mosquito hemolymph CLIPB4 KD lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. Then the membrane was incubated with rabbit anti-CLIPB4 antigen affinity purified monoclonal antibody (DZ33989-1) at 1:3000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody at a dilution of 1:15000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with Tanon 5200 system. A specific band was detected for CLIPB4 at approximately 40 kDa. The expected band size for CLIPB4 is at 39 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mosquito hemolymph β-galactosidase KD lysates,
Lane 2: mosquito hemolymph CLIPB4 KD lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. Then the membrane was incubated with rabbit anti-CLIPB4 antigen affinity purified monoclonal antibody (DZ33989-1) at 1:3000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody at a dilution of 1:15000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with Tanon 5200 system. A specific band was detected for CLIPB4 at approximately 40 kDa. The expected band size for CLIPB4 is at 39 kDa.
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Anti-African malaria mosquito CLIPB4 Antibody
CLIPB4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| African malaria mosquito | Rabbit | WB |
Tested Applications
CLIPB4 antibody was positively detected by WB in:
mosquito hemolymph β-galactosidase, mosquito hemolymph CLIPB4 KD
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Product Information
Product Name
Anti-African malaria mosquito CLIPB4 Antibody
View all CLIPB4 Antibodies
View all CLIPB4 Antibodies
Form
Liquid
Uniprot
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Observed Molecular Weight
40 kDa
Cite This Product
Anti-African malaria mosquito CLIPB4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ33989-1)
Protocols
Western Blot protocol for CLIPB4 antibody
Review
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1 Review For Anti-African malaria mosquito CLIPB4 Antibody
The CLIPB4 antibody was efficiently used to detect CLIPB4 protein secreted in the mosquito hemolymph. The protein migrates at ~40 kDa in SDS-PAGE.
| SKU | DZ33989-1 |
|---|---|
| Application | Western Blot |
| Sample | β-galactosidase gene knockdown mosquito hemolymph, CLIPB4 knockdown mosquito hemolymph |
| Primary Incubation | Overnight at 4°C |
| Secondary Incubation | For 1 hour at room temperature |
| Secondary Antibody Dilution | 1:3000 |
| Detection | Clarity Max Western ECL substrate |
| Results Summary | Image showing the specificity of CLIPB4 antibody. Lane 1 includes hemolymph extracted from mosquitoes silenced for the bacterial β-galactosidase gene. Lane 2 contains hemolymph extracted from mosquitoes silenced for the CLIPB4 gene. |
Dr. V, Professor of Vector Biology, Biology Department, American University of Beirut
Verified customer
Submitted 2025-04-08
Product Promise
We promise Anti-African malaria mosquito CLIPB4 Antibody performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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DZ33989-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026