Anti-AMPK Alpha 1/PRKAA1 (Phospho-T183)/AMPK Alpha 2/PRKAA2 (Phospho-T172) Antibody (Monoclonal, 31P05)

Product Name

Anti-AMPK Alpha 1/PRKAA1 (Phospho-T183)/AMPK Alpha 2/PRKAA2 (Phospho-T172) Antibody (Monoclonal, 31P05)

View all PRKAA1/PRKAA2 Antibodies

SKU/Catalog Number

M00994-3

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-AMPK Alpha 1/PRKAA1 (Phospho-T183)/AMPK Alpha 2/PRKAA2 (Phospho-T172) Antibody (Monoclonal, 31P05) catalog # M00994-3. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AMPK Alpha 1/PRKAA1 (Phospho-T183)/AMPK Alpha 2/PRKAA2 (Phospho-T172) Antibody (Monoclonal, 31P05) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00994-3)

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

31P05

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr183 of human AMPK alpha 1.

Reactive Species

M00994-3 is reactive to PRKAA1/PRKAA2 in Human, Mouse, Rat

Background of PRKAA1/PRKAA2

5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00994-3 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunohistochemistry, 1:50-200

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information PRKAA1/PRKAA2

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