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Product Info Summary
| SKU: | M33909 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hu CD165 Purified Monoclonal Antibody
SKU/Catalog Number
M33909
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD165 Purified Monoclonal Antibody (Catalog# M33909). Tested in Flow Cytometry, IP, WB, IHC-F, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD165 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M33909)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
SN2
Isotype
Mouse IgG1 kappa
Immunogen
CD165 purified from human Molt-4 cell line. The mouse monoclonal antibody SN2, also known as SN2 N6-D11, recognizes an extracellular epitope of CD165, an approximately 37-42 kDa transmembrane glycoprotein expressed mainly on leukemic T cells, double positive and double negative thymocytes (CD4-CD8-, CD4+CD8+), and platelets.
Cross-reactivity
Cross-reactivity with LARGE2 was not determined.
Reactive Species
M33909 is reactive to CD165 in Human
Observed Molecular Weight
34-48 kDa
Calculated molecular weight
46.7 kDa
Background of CD165
CD165 is a poorly characterized transmembrane protein highly expressed on platelets and many leukemic T cell lines. At lower level it is expressed on a proportion of circulating T cells and monocytes, on thymic epithelium, fibroblasts, epidermal keratinocytes, pancreatic islet cells, and some neurons. It might have a role in adhesion between thymocytes and thymic epithelial cells and it can be used as a marker for tumor progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M33909 is guaranteed for Flow Cytometry, IP, IHC-F, ICC, WB Boster Guarantee
Recommend Dilution
Immunohistochemistry (frozen sections): Application note: acetone fixation.
Flow cytometry: Recommended dilution: 1-4 µg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.
Validation Images & Assay Conditions
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Separation of Jurkat cells stained using anti-human CD165 (SN2) purified antibody (concentration in sample 0,6 µg/ml, GAM APC, red) from Jurkat cells unstained by primary antibody (GAM APC, black) in flow cytometry analysis (surface staining).
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Anti-Hu CD165 Purified (clone SN2) works in WB application under non-reducing conditions.
Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Molt-4, Raji, and Hep-G2 cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.
Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody SN2 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.
CD165 molecules were detected at ~34-48 kDa in Jurkat and Molt-4 cell lines under non-reducing conditions.
Specific Publications For Anti-Hu CD165 Purified Monoclonal Antibody (M33909)
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