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Product Info Summary
| SKU: | A32244 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PRKCDBP CAVIN3 Antibody
SKU/Catalog Number
A32244
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PRKCDBP CAVIN3 Antibody (Catalog # A32244). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
PRKCDBP antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-PRKCDBP CAVIN3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32244)
Host
Rabbit
Contents
PRKCDBP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal PRKCDBP antibody was raised against an 17 amino acid peptide near the center of human PRKCDBP. The immunogen is located within amino acids 150 - 200 of PRKCDBP.
Cross-reactivity
It is predicted to not cross-react with other members of the cavin family.
Reactive Species
A32244 is reactive to PRKCDBP in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
27.7 kDa
Background of PRKCDBP
The protein kinase C delta (PKC-δ) binding protein (PRKCDBP), also known as cavin-3, is a member of the cavin family of proteins that are involved in caveolin formation and regulation. PRKCDBP was initially identified in a screen of cultured cell lines for proteins that were strongly induced by serum starvation. Studies indicate that PRKCDBP binds not only to PKC-δ but also to caveolin-1 and helps regulate caveolin traffic and function. Similar to other members of the cavin family, the expression of PRKCDBP was found to be down-regulated in various cancer cell lines, suggesting a possible tumor suppressor function of PRKCDBP.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32244 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PRKCDBP antibody can be used for detection of PRKCDBP by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PRKCDBP in A20 cell lysate with PRKCDBP antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of PRKCDBP in human spleen tissue with PRKCDBP antibody at 2.5 μg/ml.
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Immunofluorescence of PRKCDBP in human spleen tissue with PRKCDBP antibody at 20 μg/ml.
Specific Publications For Anti-PRKCDBP CAVIN3 Antibody (A32244)
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