Click image to see more details
Product Info Summary
| SKU: | M06819-25 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB, Luminex |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Acetyl-Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM169
SKU/Catalog Number
M06819-25
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Acetyl-Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM169 (Catalog # M06819-25). Tested in WB, ELISA, Luminex, ICC applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Acetyl-Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM169 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06819-25)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM169
Isotype
Rabbit IgG
Immunogen
An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys23).
Cross-reactivity
This antibody reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross-reactivity with other acetylated Lysines in histone H3.
Reactive Species
M06819-25 is reactive to H3F3A in Human, Vertebrates
Calculated molecular weight
15.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06819-25 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot: 0.5 ug/mL - 2 ug/mL
ELISA: 0.5 ug/mL - 1 ug/mL
Luminex: 0.2 ug/mL – 1 ug/mL
Immunocytochemistry: 1 ug/mL- 2 ug/mL.
Validation Images & Assay Conditions
Click image to see more details
Western Blotting result
Western Blot of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2), and recombinant histone H3.3 (3), using RM169 at 1 ug/mL, showed a band of histone H3 acetylated at Lysine 23 in treated HeLa.
Click image to see more details
Specificity Test result
RM169 specifically reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with unmodified Lysine 23 (K23 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
Click image to see more details
ICC result
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H3 (Lys23) (Clone RM169) (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
Specific Publications For Anti-Acetyl-Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM169 (M06819-25)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Acetyl-Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM169?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Acetyl-Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM169
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
