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Product Info Summary
| SKU: | M06819-27 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | Dot blot, WB, Luminex |
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Product info
Product Name
Anti-Crotonyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM339
SKU/Catalog Number
M06819-27
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Crotonyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM339 (Catalog # M06819-27). Tested in WB, Dot blot, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Crotonyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM339 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06819-27)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM339
Isotype
Rabbit IgG
Immunogen
A crotonyl-peptide corresponding to Crotonyl-Histone H3 (Lys9).
Cross-reactivity
This antibody reacts to Histone H3 crotonylated at Lysine 9 (K9cr), and does not cross-react with acetylated or butyrylated Lysine 9. No cross-reactivity with other crotonylated Lysines in histone H3
Reactive Species
M06819-27 is reactive to H3F3A in Human, Vertebrates
Calculated molecular weight
15.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06819-27 is guaranteed for Dot blot, WB, Luminex Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1 ug/mL – 5 ug/mL
DotBlot: 0.5 ug/mL – 2 ug/mL
Luminex: 0.05 ug/mL – 0.5 ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot using Anti-Histone H3K9cr Rabbit Monoclonal Antibody RM339 against H3K9cr [Crotonyl-Histone H3 (Lys9)]. Anti-Histone H3 and anti-G6PDH were used as controls. A crotonylation inducing metabolite was used to increase the H3K9cr signal.
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Dot Blot result
A Peptide dotblot showing Anti-Histone H3K9cr Rabbit Monoclonal Antibody RM339 reacts specifically to Histone H3 crotonylated at Lysine 9 (H3K9-Crotonyl), and RM339
Specific Publications For Anti-Crotonyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM339 (M06819-27)
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