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Product Info Summary
| SKU: | M08865 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB, Luminex |
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Product info
Product Name
Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody, Clone#RM186
SKU/Catalog Number
M08865
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody, Clone#RM186 (Catalog # M08865). Tested in WB, ELISA, Luminex, ICC applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody, Clone#RM186 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08865)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM186
Isotype
Rabbit IgG
Immunogen
An unmodified peptide corresponding to the N-terminus of Histone H3.
Cross-reactivity
This antibody recognizes Histone H3 that is unmodified at Lys4 but does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.
Reactive Species
M08865 is reactive to HIST3H3 in Human, Vertebrates
Calculated molecular weight
15.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08865 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot: 1 ug/mL – 2 ug/mL
ELISA: 0.5 ug/mL - 1 ug/mL
Luminex: 0.5 ug/mL – 2 ug/mL
Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of histone H3 with unmodified Lys4.
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ICC result
Immunocytochemical staining of HeLa cells using Anti-Histone H3 (Unmodified Lys4) (Clone RM186) (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
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Specificity Test result
RM186 specifically recognizes Histone H3 that is unmodified at Lys4 and does not recognize acetylated, monomethylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.
Specific Publications For Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody, Clone#RM186 (M08865)
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Customer Q&As
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6 Customer Q&As for Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody, Clone#RM186
Question
I would like to test anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal antibody, Clone#RM186 M08865 on human right testis for research purposes, then I may be interested in using anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal antibody, Clone#RM186 M08865 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
The products we sell, including anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal antibody, Clone#RM186 M08865, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-20
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right testis using anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal antibody, Clone#RM186 M08865. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
I was wanting to use your anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal antibody, Clone#RM186 for WB for human right testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right testis identification?
K. Carter
Verified customer
Asked: 2019-10-11
Answer
As indicated on the product datasheet, M08865 anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal antibody, Clone#RM186 has been validated for ELISA, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human right testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-11
Question
Can you help my question with product M08865, anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal antibody, Clone#RM186. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M08865 anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal antibody, Clone#RM186, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-12
Question
Would anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal antibody, Clone#RM186 M08865 work for WB with right testis?
F. Zhao
Verified customer
Asked: 2018-09-17
Answer
According to the expression profile of right testis, HIST3H3 is highly expressed in right testis. So, it is likely that anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal antibody, Clone#RM186 M08865 will work for WB with right testis.
Boster Scientific Support
Answered: 2018-09-17
Question
We are currently using anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal antibody, Clone#RM186 M08865 for rat tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
T. Wu
Verified customer
Asked: 2014-10-02
Answer
The anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal antibody, Clone#RM186 (M08865) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-02
