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Product Info Summary
| SKU: | A33947S10 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat, Drosophila melanogaster |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Histone H3 (S10) Antibody
SKU/Catalog Number
A33947S10
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Histone H3 (S10) Antibody catalog # A33947S10. Tested in WB, IHC, Flow Cytometry, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Drosophila melanogaster.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Histone H3 (S10) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A33947S10)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Ser10. AA range:1-50
Reactive Species
A33947S10 is reactive to H3C1 in Human, Mouse, Rat, Drosophila melanogaster
Calculated molecular weight
25.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A33947S10 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ICC/IF 1:200-1:1000
ELISA 1:10000
FC 1:500
Validation Images & Assay Conditions
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Western Blot analysis of KB cells using Phospho-Histone H3 (S10) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from NIH/3T3 cells and HepG2 cells, using Histone H3.1 (Phospho-Ser10) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Histone H3.1 (Phospho-Ser10) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunofluorescence analysis of Hela cell. 1, Histone H3 (phospho Ser10) Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). β-Tubulin Monoclonal Antibody (5G3) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
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Western Blot analysis of various cells using Phospho-Histone H3 (S10) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Specific Publications For Anti-Phospho-Histone H3 (S10) Antibody (A33947S10)
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