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Product Info Summary
| SKU: | A07182 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-G2A GPR132 Antibody
SKU/Catalog Number
A07182
Size
100μl
Form
Liquid
Description
Boster Bio Anti-G2A GPR132 Antibody catalog # A07182. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-G2A GPR132 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07182)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR132. AA range:293-342
Reactive Species
A07182 is reactive to GPR132 in Human, Monkey, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
42.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07182 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
IF 1:100-300
Validation Images & Assay Conditions
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Western Blot analysis of 293T cells using G2A Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from COS7 cells, using GPR132 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min).3, Picture B: DAPI (blue) 10min.
Specific Publications For Anti-G2A GPR132 Antibody (A07182)
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Customer Reviews
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5 Customer Q&As for Anti-G2A GPR132 Antibody
Question
I was wanting to use your anti-G2A antibody for WB for mouse synovium thymus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse synovium thymus identification?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
It shows on the product datasheet, A07182 anti-G2A antibody has been tested for WB on human, monkey, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse synovium thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-11
Question
Will A07182 anti-G2A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
It shows on the product datasheet, A07182 anti-G2A antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-03
Question
Here is the WB image, lot number and protocol we used for synovium thymus using anti-G2A antibody A07182. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-03
Question
Would anti-G2A antibody A07182 work for WB with synovium thymus?
R. Johnson
Verified customer
Asked: 2015-02-20
Answer
According to the expression profile of synovium thymus, GPR132 is highly expressed in synovium thymus. So, it is likely that anti-G2A antibody A07182 will work for WB with synovium thymus.
Boster Scientific Support
Answered: 2015-02-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for synovium thymus using anti-G2A antibody A07182. Let me know if you need anything else.
G. Dhar
Verified customer
Asked: 2013-08-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-30
