Product Info Summary
| SKU: | PA1854 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FES Antibody Picoband®
SKU/Catalog Number
PA1854
BA3831-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FES Antibody catalog # PA1854. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FES Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1854)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human FES.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1854 is reactive to FES in Human
Observed Molecular Weight
93 kDa
Calculated molecular weight
93.5 kDa
Background of FES
FES (feline sarcoma oncogene) is an enzyme that in humans is encoded by the FES gene, also known as Proto-oncogene tyrosine-protein kinase Fes/Fps, Feline sarcoma/Fujinami avian sarcoma oncogene homolog, Proto-oncogene c-Fes, Proto-oncogene c-Fps, p93c-fes c-fes/fps protein, FPS, Oncogene FES, feline sarcoma virus, FPS. This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. Non-onc intervening sequences were present in the human counterpart. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia, but it is also involved in normal hematopoiesis. A truncated transcript has been identified that is generated utilizing a start site in one of the far downstream exons but a protein product associated with this transcript has not been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1854 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-FES antibody, PA1854, Western blotting
All lanes: Anti FES (PA1854) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 93KD
Observed bind size: 93KD
Specific Publications For Anti-FES Antibody Picoband® (PA1854)
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Customer Q&As
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5 Customer Q&As for Anti-FES Antibody Picoband®
Question
I see that the anti-FES antibody PA1854 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-11
Question
My lab would like to test anti-FES antibody PA1854 on human tongue for research purposes, then I may be interested in using anti-FES antibody PA1854 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The products we sell, including anti-FES antibody PA1854, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-19
Question
Will PA1854 anti-FES antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Dhar
Verified customer
Asked: 2017-01-25
Answer
You can see on the product datasheet, PA1854 anti-FES antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-25
Question
Would anti-FES antibody PA1854 work for WB with tongue?
S. Walker
Verified customer
Asked: 2014-08-05
Answer
According to the expression profile of tongue, FES is highly expressed in tongue. So, it is likely that anti-FES antibody PA1854 will work for WB with tongue.
Boster Scientific Support
Answered: 2014-08-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tongue using anti-FES antibody PA1854. Let me know if you need anything else.
A. Lewis
Verified customer
Asked: 2014-06-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-06-04


