Anti-Hepatitis Virus A59 Nonstructural Protein 9 1a Monoclonal Antibody

Product Name

Anti-Hepatitis Virus A59 Nonstructural Protein 9 1a Monoclonal Antibody

View all 1A Antibodies

SKU/Catalog Number

M19765

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Hepatitis Virus A59 Nonstructural Protein 9 1a Monoclonal Antibody (Catalog # M19765). Tested in IF, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Hepatitis Virus A59 Nonstructural Protein 9 1a Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M19765)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 2C6.H1 IgG2b kappa

Isotype

IgG2b kappa

Immunogen

This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein.  This protein is part of the viral replicase polyprotein.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M19765 is reactive to 1A in Mouse

Observed Molecular Weight

42 kDa

Background of 1A

The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M19765 is guaranteed for IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF Microscopy: 1:1,000
WB: 1:1,000
This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3% PFA.  After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.

Validation Images & Assay Conditions

See full target information 1A

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