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Product Info Summary
| SKU: | A07544 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Homeobox protein Hox-D3 HoxD3 Antibody
SKU/Catalog Number
A07544
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Homeobox protein Hox-D3 HoxD3 Antibody catalog # A07544. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Homeobox protein Hox-D3 HoxD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07544)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human HOXD3. AA range:211-260
Reactive Species
A07544 is reactive to HOXD3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
45.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07544 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
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Western blot analysis of lysate from HepG2 cells, using HOXD3 Antibody.
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Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
Specific Publications For Anti-Homeobox protein Hox-D3 HoxD3 Antibody (A07544)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Homeobox protein Hox-D3 HoxD3 Antibody
Question
We want to test anti-HoxD3 antibody A07544 on rat kidney for research purposes, then I may be interested in using anti-HoxD3 antibody A07544 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
The products we sell, including anti-HoxD3 antibody A07544, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-27
Question
Is there a BSA free version of anti-HoxD3 antibody A07544 available?
Verified Customer
Verified customer
Asked: 2019-03-05
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-HoxD3 antibody A07544 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-05
Question
Is this A07544 anti-HoxD3 antibody reactive to the isotypes of HOXD3?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
The immunogen of A07544 anti-HoxD3 antibody is Synthesized peptide derived from the Internal region of human HoxD3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-26
Question
Does anti-HoxD3 antibody A07544 work for IHC with kidney?
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
According to the expression profile of kidney, HOXD3 is highly expressed in kidney. So, it is likely that anti-HoxD3 antibody A07544 will work for IHC with kidney.
Boster Scientific Support
Answered: 2017-08-07
Question
We are currently using anti-HoxD3 antibody A07544 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
K. Rodriguez
Verified customer
Asked: 2017-03-07
Answer
The anti-HoxD3 antibody (A07544) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-07
Question
I see that the anti-HoxD3 antibody A07544 works with IHC, what is the protocol used to produce the result images on the product page?
N. Johnson
Verified customer
Asked: 2015-06-02
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-06-02
