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Product Info Summary
| SKU: | A07156 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Homeobox protein Hox-B5 HoxB5 Antibody
SKU/Catalog Number
A07156
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Homeobox protein Hox-B5 HoxB5 Antibody catalog # A07156. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Homeobox protein Hox-B5 HoxB5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07156)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human HoxB5. AA range:150-199
Reactive Species
A07156 is reactive to HOXB5 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
29.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07156 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blot analysis of lysate from HUVEC cells, using HoxB5 antibody.
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Western Blot analysis of various cells using HoxB5 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Specific Publications For Anti-Homeobox protein Hox-B5 HoxB5 Antibody (A07156)
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Customer Q&As
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3 Customer Q&As for Anti-Homeobox protein Hox-B5 HoxB5 Antibody
Question
Do you have a BSA free version of anti-HoxB5 antibody A07156 available?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HoxB5 antibody A07156 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-16
Question
Would A07156 anti-HoxB5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
You can see on the product datasheet, A07156 anti-HoxB5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-19
Question
We are currently using anti-HoxB5 antibody A07156 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
T. Dhar
Verified customer
Asked: 2016-07-08
Answer
The anti-HoxB5 antibody (A07156) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-08
