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Product Info Summary
| SKU: | M13250 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-IRG1/ACOD1 Antibody (Monoclonal, 33A14)
SKU/Catalog Number
M13250
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-IRG1/ACOD1 Antibody (Monoclonal, 33A14) catalog # M13250. Tested in WB, ICC/IF applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRG1/ACOD1 Antibody (Monoclonal, 33A14) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13250)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
33A14
Immunogen
Recombinant protein within mouse IRG1 aa 3-486.
Reactive Species
M13250 is reactive to Acod1 in Mouse
Observed Molecular Weight
54 kDa
Calculated molecular weight
53.8 kDa
Background of Acod1
Enables aconitate decarboxylase activity and protein homodimerization activity. Involved in several processes, including cellular response to cytokine stimulus; regulation of defense response; and regulation of gene expression. Acts upstream of or within response to lipopolysaccharide. Located in mitochondrion. Orthologous to human ACOD1 (aconitate decarboxylase 1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13250 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunocytochemistry/Immunofluorescence, 1:50-200
Positive Control
WB: human THP-1 whole cell lysates, human THP-1+LPS whole cell lysates
Validation Images & Assay Conditions
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Western blot analysis of IRG1/ACOD1 using anti-IRG1/ACOD1 antibody (M13250).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human THP-1+LPS whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRG1/ACOD1 antigen affinity purified monoclonal antibody (M13250) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IRG1/ACOD1 at approximately 54 kDa. The expected band size for IRG1/ACOD1 is at 54 kDa.
Specific Publications For Anti-IRG1/ACOD1 Antibody (Monoclonal, 33A14) (M13250)
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