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Product Info Summary
| SKU: | RP1056 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-KAT13A/SRC1/NCOA1 Antibody Picoband®
SKU/Catalog Number
RP1056
PB0305 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KAT13A/SRC1/NCOA1 Antibody catalog # RP1056. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KAT13A/SRC1/NCOA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1056)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human KAT13A recombinant protein (Position: H614-Q826). Human KAT13A shares 92% amino acid (aa) sequence identity with mouse KAT13A.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1056 is reactive to NCOA1 in Human, Mouse, Rat
Observed Molecular Weight
157 kDa
Calculated molecular weight
156.8 kDa
Background of NCOA1
The nuclear receptor coactivator 1 (NCOA1), also known as SRC1, is a transcriptional coregulatory protein that contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. NCOA1 is recruited to DNA promotion sites by ligand-activated nuclear receptors. NCOA1, in turn, acylates histones, which makes downsteam DNA more accessible to transcription. Hence, NCOA1 assists nuclear receptors in the upregulation of DNA expression. It has been found that NCOA1 can enhance the transcriptional activity of ligand-bound PGR but does not alter the basal activity of the target promoter. It also enhances estrogen receptor, glucocorticoid receptor, thyroid hormone receptor, and retinoid X receptor transcriptional activities through their cognate DNA response elements in the presence of hormone. What’s more, SRC1 may play a role as a bridging molecule between nuclear hormone receptors and general transcription factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1056 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of KAT13A/SRC1 using anti-KAT13A/SRC1 antibody (RP1056).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HeLa whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse NIH3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT13A/SRC1 antigen affinity purified polyclonal antibody (RP1056) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KAT13A/SRC1 at approximately 157 kDa. The expected band size for KAT13A/SRC1 is at 157 kDa.
Specific Publications For Anti-KAT13A/SRC1/NCOA1 Antibody Picoband® (RP1056)
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1 Customer Q&As for Anti-KAT13A/SRC1/NCOA1 Antibody Picoband®
Question
We are currently using anti-KAT13A/SRC1/NCOA1 antibody RP1056 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
K. Parker
Verified customer
Asked: 2016-03-30
Answer
The anti-KAT13A/SRC1/NCOA1 antibody (RP1056) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-30
