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Product Info Summary
| SKU: | A19762 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Chimpanzee |
| Host: | Chicken |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-L1/ORF2 Antibody
SKU/Catalog Number
A19762
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-L1/ORF2 Antibody (Catalog # A19762). Tested in Dot blot, ELISA, WB applications. This antibody reacts with Human, Chimpanzee.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-L1/ORF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A19762)
Host
Chicken
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgY
Immunogen
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A19762 is reactive to ORF2 in Human, Chimpanzee
Observed Molecular Weight
19 kDa
Background of ORF2
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A19762 is guaranteed for ELISA, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| ELISA | 000 | |
| WB: 1:2 | 000-1_10 | 000 |
Validation Images & Assay Conditions
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Western blot using Boster's IgY fraction of anti-L1/ORF2 antibody shows detection of induced bacterially expressed human ORF2 (left lane). No specific band staining is seen in the uninduced lane (right lane). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa corresponds to a human L1/ORF2 EN domain fusion protein (arrowhead). Personal communication, D. Symer, NCI, Bethesda, MD.
Specific Publications For Anti-L1/ORF2 Antibody (A19762)
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6 Customer Q&As for Anti-L1/ORF2 Antibody
Question
Would A19762 anti-L1/ORF2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
It shows on the product datasheet, A19762 anti-L1/ORF2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-08
Question
We are currently using anti-L1/ORF2 antibody A19762 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The anti-L1/ORF2 antibody (A19762) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-20
Question
Is a blocking peptide available for product anti-L1/ORF2 antibody (A19762)?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
We do provide the blocking peptide for product anti-L1/ORF2 antibody (A19762). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-14
Question
I see that the anti-L1/ORF2 antibody A19762 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-06-19
Question
My question regarding product A19762, anti-L1/ORF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Parker
Verified customer
Asked: 2017-01-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A19762 anti-L1/ORF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-06
Question
Do you have a BSA free version of anti-L1/ORF2 antibody A19762 available?
S. Collins
Verified customer
Asked: 2016-02-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-L1/ORF2 antibody A19762 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-03
