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Product Info Summary
| SKU: | A07108 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Nephrocystin-5 IQCB1 Antibody
SKU/Catalog Number
A07108
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Nephrocystin-5 IQCB1 Antibody catalog # A07108. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nephrocystin-5 IQCB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07108)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human IQCB1. AA range:431-480
Reactive Species
A07108 is reactive to IQCB1 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
68.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07108 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of SH-SY5Y cells using Nephrocystin-5 Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from K562 cells, using IQCB1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HepG2 cells using IQCB1 antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Specific Publications For Anti-Nephrocystin-5 IQCB1 Antibody (A07108)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Nephrocystin-5 IQCB1 Antibody
Question
Does anti-Nephrocystin-5 antibody A07108 work on canine WB with ovary?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
Our lab technicians have not validated anti-Nephrocystin-5 antibody A07108 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Nephrocystin-5 antibody A07108 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine ovary in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-03
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Nephrocystin-5 antibody A07108. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-09
Question
We are currently using anti-Nephrocystin-5 antibody A07108 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
The anti-Nephrocystin-5 antibody (A07108) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-11
