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Product Info Summary
| SKU: | M08095-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® (monoclonal, 3H6C8)
SKU/Catalog Number
M08095-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® (monoclonal, 3H6C8) catalog # M08095-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® (monoclonal, 3H6C8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08095-2)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
3H6C8
Isotype
IgG2b
Immunogen
E.coli-derived human Proteasome 20S beta 7/PSMB7 recombinant protein (Position: D17-S277).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M08095-2 is reactive to PSMB7 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
30.0 kDa
Background of PSMB7
Proteasome subunit beta type-7 as known as 20S proteasome subunit beta-2 is a protein that in humans is encoded by the PSMB7 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08095-2 is guaranteed for Flow Cytometry, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western Blot (WB) | 0.25-0.5 µg/ml | Human, Monkey, Mouse, Rat |
| Flow Cytometry (FC) | 1-3 µg/1x106 cells | Human |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of Proteasome 20S beta 7/PSMB7 using anti-Proteasome 20S beta 7/PSMB7 antibody (M08095-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Proteasome 20S beta 7/PSMB7 antigen affinity purified monoclonal antibody (Catalog # M08095-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Proteasome 20S beta 7/PSMB7 at approximately 25 kDa. The expected band size for Proteasome 20S beta 7/PSMB7 is at 30 kDa.
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Flow Cytometry analysis of CACO-2 cells using anti-PSMB7 antibody (M08095-2).
Overlay histogram showing CACO-2 cells stained with M08095-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PSMB7 Antibody (M08095-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® (monoclonal, 3H6C8) (M08095-2)
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