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Product Info Summary
| SKU: | M03826-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-DDX6 Antibody Picoband® (monoclonal, 8G6)
SKU/Catalog Number
M03826-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DDX6 Antibody Picoband® (monoclonal, 8G6) catalog # M03826-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDX6 Antibody Picoband® (monoclonal, 8G6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03826-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
8G6
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human DDX6 recombinant protein (Position: Q56-P483).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M03826-1 is reactive to DDX6 in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
54.4 kDa
Background of DDX6
DDX6 (DEAD/H BOX 6), also known as HLR2 or p54, is an enzyme that in humans is encoded by the DDX6 gene. DDX6 belongs to the DEAD box family of putative RNA helicases that contain a characteristic asp-glu-ala-asp (DEAD) box motif (Seto et al., 1995). Tunnacliffe et al. (1993) assigned the DDX6 gene more precisely using a panel of sequence tagged sites (STSs) representing 30 markers previously assigned to 11q23. Using mass spectroscopy, Fenger-Gron et al. (2005) found that RCK, EDC3 (YJDC), and HEDLS (RCD8) coimmunopurified with DCP1A and DCP2 from HEK293 cell lysates. Overexpression of DCP2, RCK, or EDC3 in HeLa cells reduced the association of endogenous DCP1A and XRN1 with cytoplasmic P bodies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03826-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human Sw620 whole cell, human Raji whole cell, human THP-1 whole cell, human Jurkat whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell
ICC/IF: MCF-7 cell
FCM: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of DDX6 using anti-DDX6 antibody (M03826-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Sw620 whole cell lysates,
Lane 4: human Raji whole cell lysates,
Lane 5: human THP-1 whole cell lysates,
Lane 6: human Jurkat whole cell lysates,
Lane 7: rat PC-12 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DDX6 antigen affinity purified monoclonal antibody (Catalog # M03826-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DDX6 at approximately 54KD. The expected band size for DDX6 is at 54KD.
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IF analysis of DDX6 using anti-DDX6 antibody (M03826-1).
DDX6 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-DDX6 Antibody (M03826-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of Hela cells using anti-DDX6 antibody (M03826-1).
Overlay histogram showing Hela cells stained with M03826-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-DDX6 Antibody (M03826-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-DDX6 Antibody Picoband® (monoclonal, 8G6) (M03826-1)
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