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Product Info Summary
| SKU: | A06442 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Replication factor C subunit 3 RFC3 Antibody
SKU/Catalog Number
A06442
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Replication factor C subunit 3 RFC3 Antibody catalog # A06442. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Replication factor C subunit 3 RFC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06442)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RFC3. AA range:178-227
Reactive Species
A06442 is reactive to RFC3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
40.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06442 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using RFC3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysate from HepG2 cells, using RFC3 antibody.
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Immunohistochemistry analysis of RFC3 antibody in paraffin-embedded human brain tissue.
Specific Publications For Anti-Replication factor C subunit 3 RFC3 Antibody (A06442)
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Customer Q&As
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7 Customer Q&As for Anti-Replication factor C subunit 3 RFC3 Antibody
Question
Does A06442 anti-RFC3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
You can see on the product datasheet, A06442 anti-RFC3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-25
Question
My lab would like to test anti-RFC3 antibody A06442 on rat embryonic kidney for research purposes, then I may be interested in using anti-RFC3 antibody A06442 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The products we sell, including anti-RFC3 antibody A06442, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-04
Question
See attached the WB image, lot number and protocol we used for embryonic kidney using anti-RFC3 antibody A06442. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-06
Question
Will anti-RFC3 antibody A06442 work for WB with embryonic kidney?
A. Baker
Verified customer
Asked: 2019-01-02
Answer
According to the expression profile of embryonic kidney, RFC3 is highly expressed in embryonic kidney. So, it is likely that anti-RFC3 antibody A06442 will work for WB with embryonic kidney.
Boster Scientific Support
Answered: 2019-01-02
Question
I see that the anti-RFC3 antibody A06442 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-11-02
Question
Is there a BSA free version of anti-RFC3 antibody A06442 available?
Verified Customer
Verified customer
Asked: 2018-08-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RFC3 antibody A06442 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-31
Question
We are currently using anti-RFC3 antibody A06442 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
The anti-RFC3 antibody (A06442) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-10
